Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ
Abstract
A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences.
- Inventors:
-
- Livermore, CA
- Tracy, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 871992
- Patent Number(s):
- 5840482
- Assignee:
- Regents of University of California (Alameda, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- chromosome; specific; nucleic; acid; probe; method; determining; situ; producing; selected; highly; repeating; sequences; described; nonspecific; binding; autosomal; chromosomes; signal; provided; inventive; primers; allowing; pcr; sample; amplification; production; dna; painting; nucleic acid; chromosome specific; acid probe; specific nucleic; repeating sequences; sample amplification; specific probe; inventive primers; primers allowing; probe production; probe selected; painting sequences; nonspecific binding; chromosome painting; specific binding; dna chromosome; highly repeating; /435/999/
Citation Formats
Gray, Joe W, and Weier, Heinz-Ulrich. Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ. United States: N. p., 1998.
Web.
Gray, Joe W, & Weier, Heinz-Ulrich. Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ. United States.
Gray, Joe W, and Weier, Heinz-Ulrich. Thu .
"Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ". United States. https://www.osti.gov/servlets/purl/871992.
@article{osti_871992,
title = {Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ},
author = {Gray, Joe W and Weier, Heinz-Ulrich},
abstractNote = {A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}
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