Title: Cellulose binding domain proteins

Abstract

A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

Inventors:

Shoseyov, Oded ^[1]; Shpiegl, Itai ^[2]; Goldstein, Marc ^[3]; Doi, Roy ^[3]

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+ Show Inventor Affiliations

1. Karmey Yosef, IL
2. Rehovot, IL
3. Davis, CA

Issue Date:

1998-01-01

Research Org.:

Univ. of California (United States)

OSTI Identifier:

871978

Patent Number(s):

5837814

Assignee:

Yissum Research Development Co. of Hebrew University Of Jeruslame (IL); University of CA (CA)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A01 - AGRICULTURE A01N - PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

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A - HUMAN NECESSITIES A01 - AGRICULTURE A01N - PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF

A01N25/10 - Macromolecular compounds
A01N63/50 - Isolated enzymes

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K38/00 - Medicinal preparations containing peptides
A61K47/62 - the modifying agent being a protein, peptide or polyamino acid

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/00 - Peptides having more than 20 amino acids
C07K14/33 - from Clostridium
C07K16/065 - {Purification, fragmentation}
C07K2319/20 - containing a tag with affinity for a non-protein ligand
C07K2319/35 - containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
C07K2319/705 - containing a protein-A fusion

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/62 - DNA sequences coding for fusion proteins
C12N5/0025 - {Culture media for plant cell or plant tissue culture}
C12N9/2434 - {acting on beta-1,4-glucosidic bonds}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y302/01004 - Cellulase

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/548 - Carbohydrates, e.g. dextran
G01N33/566 - using specific carrier or receptor proteins as ligand binding reagents {where possible specific carrier or receptor proteins are classified with their target compounds}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S436/827 - Lectins
Y10S436/828 - Protein A
Y10S530/82 - Proteins from microorganisms
Y10S530/861 - involving IgG3, IgG4, IgA, or IgY

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DOE Contract Number:  

FG03-92ER20069

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

cellulose; binding; domain; proteins; cbd; affinity; crystalline; chitin; disclosed; methods; molecular; cloning; recombinant; production; fusion; products; comprising; protein; likewise; described; wide; range; applications; contemplated; including; drug; delivery; separations; diagnostic; techniques; molecular cloning; affinity separations; crystalline cellulose; including drug; likewise described; diagnostic techniques; binding domain; cellulose binding; wide range; products comprising; drug delivery; fusion products; recombinant production; domain proteins; /530/