Method for improved selectivity in photo-activation and detection of molecular diagnostic agents

Wachter, Eric A; Fisher, Walter G; Dees, H Craig

Title: Method for improved selectivity in photo-activation and detection of molecular diagnostic agents

Abstract

A method for the imaging of a particular volume of plant or animal tissue, wherein the plant or animal tissue contains at least one photo-active molecular agent. The method includes the steps of treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of the photo-active molecular agent contained in the particular volume of the plant or animal tissue, photo-activating at least one of the at least one photo-active molecular agent in the particular volume of the plant or animal tissue, thereby producing at least one photo-activated molecular agent, wherein the at least one photo-activated molecular agent emits energy, detecting the energy emitted by the at least one photo-activated molecular agent, and producing a detected energy signal which is characteristic of the particular volume of plant or animal tissue. The present invention is also a method for the imaging of a particular volume of material, wherein the material contains at least one photo-active molecular agent.

Inventors:

Wachter, Eric A ^[1]; Fisher, Walter G ^[2]; Dees, H Craig ^[2]

Oak Ridge, TN
Knoxville, TN

Issue Date:: 1998-01-01

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

OSTI Identifier:: 871959

Patent Number(s):: 5832931

Assignee:: Photogen, Inc. (Oak Ridge, TN)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS
A61B2017/00057 - {Light}

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A61K41/008 - {Two-Photon or Multi-Photon PDT, e.g. with upconverting dyes or photosensitisers}
A61K49/0043 - {Fluorescein, used in vivo}
A61K49/0039 - {Coumarin dyes}

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61N - ELECTROTHERAPY
A61N5/062 - {Photodynamic therapy, i.e. excitation of an agent}

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
B82Y15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A61K49/0017 - {Fluorescence in vivo}

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DOE Contract Number:: AC05-84OR21400

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: method; improved; selectivity; photo-activation; detection; molecular; diagnostic; agents; imaging; particular; volume; plant; animal; tissue; contains; photo-active; agent; steps; treating; light; sufficient; promote; simultaneous; two-photon; excitation; contained; photo-activating; producing; photo-activated; emits; energy; detecting; emitted; detected; signal; characteristic; material; two-photon excitation; photo-activated molecular; simultaneous two-photon; photo-active molecular; molecular agent; animal tissue; particular volume; improved selectivity; energy emitted; light sufficient; material contains; diagnostic agent; diagnostic agents; /128/600/

Citation Formats


                    Wachter, Eric A, Fisher, Walter G, and Dees, H Craig. Method for improved selectivity in photo-activation and detection of molecular diagnostic agents.  United States: N. p., 1998. 
        Web.

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                    Wachter, Eric A, Fisher, Walter G, & Dees, H Craig. Method for improved selectivity in photo-activation and detection of molecular diagnostic agents.  United States.

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                    Wachter, Eric A, Fisher, Walter G, and Dees, H Craig. Thu .  
        "Method for improved selectivity in photo-activation and detection of molecular diagnostic agents".  United States.  https://www.osti.gov/servlets/purl/871959.

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@article{osti_871959,

  title        = {Method for improved selectivity in photo-activation and detection of molecular diagnostic agents},

  author       = {Wachter, Eric A and Fisher, Walter G and Dees, H Craig},

  abstractNote = {A method for the imaging of a particular volume of plant or animal tissue, wherein the plant or animal tissue contains at least one photo-active molecular agent. The method includes the steps of treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of the photo-active molecular agent contained in the particular volume of the plant or animal tissue, photo-activating at least one of the at least one photo-active molecular agent in the particular volume of the plant or animal tissue, thereby producing at least one photo-activated molecular agent, wherein the at least one photo-activated molecular agent emits energy, detecting the energy emitted by the at least one photo-activated molecular agent, and producing a detected energy signal which is characteristic of the particular volume of plant or animal tissue. The present invention is also a method for the imaging of a particular volume of material, wherein the material contains at least one photo-active molecular agent.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {1}

}

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Methods for improved selectivity in photo-activation and detection of molecular diagnostic agents

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Title: Method for improved selectivity in photo-activation and detection of molecular diagnostic agents

Abstract

Citation Formats

Two-step laser activation of hematoporphyrin derivative journal, April 1982

Dispersion of the two-photon cross section in rhodamine dyes journal, October 1972

A simple conceptual model for two‐photon absorption journal, January 1986

Multiphoton Spectroscopy journal, October 1967

Mechanistic investigation of doxycycline photosensitization by picosecond-pulsed and continuous wave laser irradiation of cells in culture. journal, April 1990

Über Elementarakte mit zwei Quantensprüngen journal, January 1931

A study on the possible involvement of nonlinear mechanism of light absorption by HpD with Nd: YAG laser journal, January 1986

Limitations Arising from Optical Saturation in Fluorescence and Thermal Lens Spectrometries Using Pulsed Laser Excitation: Application to the Determination of the Fluorescence Quantum Yield of Rhodamine 6G journal, December 1996

Two Photons Shine In 3-D Data Storage: Organic dyes show promise for computer memories, cancer therapy, microscopy journal, September 1996

Photodynamic Therapy Using Pheophorbide a and Nd:YAG Laser journal, January 1991

Excited State Symmetry Assignment Through Polarized Two‐Photon Absorption Studies of Fluids journal, September 1971

Threshold dependence of laser‐induced optical breakdown on pulse duration journal, March 1995

A review of the optical properties of biological tissues journal, January 1990

The effect of spatial and temporal laser beam characteristics on two-photon absorption journal, August 1975

In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indices journal, January 1995

Two-Photon Excitation in Ca F 2 : Eu 2 + journal, September 1961

Photochemistry and Photobiology of Psoralens journal, June 1979

Molecular model for solid-state polymerization of nylon 6. II. An improved model journal, July 1994

The two-photon laser-induced fluorescence of the tumor-localizing photosensitizer hematoporphyrin derivative. Resonance-enhanced 750 nm two-photon excitation into the near-UV Soret band. journal, September 1986

p-Bis(o-methylstyryl)benzene as a power-squared sensor for two-photon absorption measurements between 537 and 694 nm journal, November 1986

1993 Syntex Award Lecture Photomedicine and photodynamic therapy journal, April 1994

Two-Photon Excitation Spectra of Polycyclic Aromatic Hydrocarbons journal, November 1980

Very high detectability in two-photon spectroscopy journal, May 1990

Second harmonic detection of sinusoidally modulated two-photon excited fluorescence journal, October 1990

60-fsec pulse generation from a self-mode-locked Ti:sapphire laser journal, January 1991

Tunable dye laser pulse converter for production of picosecond pulses journal, October 1974

Clinical and preclinical photodynamic therapy journal, January 1995

Polarization Dependence of the Two‐Photon Absorption of Tumbling Molecules with Application to Liquid 1‐Chloronaphthalene and Benzene journal, July 1970

Femtosecond Dynamics of Excited-State Evolution in [Ru(bpy)3]2+ journal, January 1997

The physics of photodynamic therapy journal, April 1986

Photodynamic Therapy with a Diode Laser for Implanted Fibrosarcoma in Mice Employing Mono-L-Aspartyl Chlorin E 6 journal, October 1996

Photophysical and Photobiological Properties of Diporphyrin Ethers journal, April 1991

Basic study on photochemical effect of pheophorbide a irradiated by Nd:YAG laser light [Nd: YAGレーザー光照射によるフェオフォーバイドaの光化学効果の基礎的研究] journal, January 1986

Photodynamic therapy using pheophorbide-a and Q-switched Nd:YAG Laser on implanted human hepatocellular carcinoma journal, October 1991

The two-photon induced fluorescence of the tumor localizing photosensitizer hematoporphyrin derivative via 1064 NM photons from a 20 NS Q-switched Nd-YAG laser journal, January 1985

In vivo EXCITATION OF PHOTOSENSITIZERS BY INFRARED LIGHT journal, August 1995

Spectroscopic diagnosis of esophageal cancer: New classification model, improved measurement system journal, June 1995

Photophysical properties of the photosensitizer pheophorbide a studied at high photon flux densities journal, February 1993

Two-Photon Excitation of 4'-Hydroxymethyl-4,5',8-Trimethylpsoralen journal, January 1997

Two-photon molecular spectroscopy journal, May 1974

Photoradiation Therapy. II. Cure of Animal Tumors With Hematoporphyrin and Light23 journal, July 1975

Psoralens as Photoactive Probes of Nucleic Acid Structure and Function: Organic Chemistry, Photochemistry, and Biochemistry journal, June 1985

Pulse generation in a cw dye laser by mode−locked synchronous pumping journal, January 1975

Two-photon excited molecular fluorescence in optically dense media journal, November 1977

Second harmonic detection of spatially filtered two-photon excited fluorescence journal, March 1993

Properties and Applications of Photodynamic Therapy journal, October 1989

Experimental investigation of ultrashort pulse laser-induced breakdown thresholds in aqueous media journal, April 1996

Two-step laser activation of hematoporphyrin derivative
journal, April 1982

Dispersion of the two-photon cross section in rhodamine dyes
journal, October 1972

A simple conceptual model for two‐photon absorption
journal, January 1986

Multiphoton Spectroscopy
journal, October 1967

Mechanistic investigation of doxycycline photosensitization by picosecond-pulsed and continuous wave laser irradiation of cells in culture.
journal, April 1990

Über Elementarakte mit zwei Quantensprüngen
journal, January 1931

A study on the possible involvement of nonlinear mechanism of light absorption by HpD with Nd: YAG laser
journal, January 1986

Limitations Arising from Optical Saturation in Fluorescence and Thermal Lens Spectrometries Using Pulsed Laser Excitation: Application to the Determination of the Fluorescence Quantum Yield of Rhodamine 6G
journal, December 1996

Two Photons Shine In 3-D Data Storage: Organic dyes show promise for computer memories, cancer therapy, microscopy
journal, September 1996

Photodynamic Therapy Using Pheophorbide a and Nd:YAG Laser
journal, January 1991

Excited State Symmetry Assignment Through Polarized Two‐Photon Absorption Studies of Fluids
journal, September 1971

Threshold dependence of laser‐induced optical breakdown on pulse duration
journal, March 1995

A review of the optical properties of biological tissues
journal, January 1990

The effect of spatial and temporal laser beam characteristics on two-photon absorption
journal, August 1975

In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indices
journal, January 1995

Two-Photon Excitation in Ca $F_{2}$ : ${Eu}^{2 +}$
journal, September 1961

Photochemistry and Photobiology of Psoralens
journal, June 1979

Molecular model for solid-state polymerization of nylon 6. II. An improved model
journal, July 1994

The two-photon laser-induced fluorescence of the tumor-localizing photosensitizer hematoporphyrin derivative. Resonance-enhanced 750 nm two-photon excitation into the near-UV Soret band.
journal, September 1986

p-Bis(o-methylstyryl)benzene as a power-squared sensor for two-photon absorption measurements between 537 and 694 nm
journal, November 1986

1993 Syntex Award Lecture Photomedicine and photodynamic therapy
journal, April 1994

Two-Photon Excitation Spectra of Polycyclic Aromatic Hydrocarbons
journal, November 1980

Very high detectability in two-photon spectroscopy
journal, May 1990

Second harmonic detection of sinusoidally modulated two-photon excited fluorescence
journal, October 1990

60-fsec pulse generation from a self-mode-locked Ti:sapphire laser
journal, January 1991

Tunable dye laser pulse converter for production of picosecond pulses
journal, October 1974

Clinical and preclinical photodynamic therapy
journal, January 1995

Polarization Dependence of the Two‐Photon Absorption of Tumbling Molecules with Application to Liquid 1‐Chloronaphthalene and Benzene
journal, July 1970

Femtosecond Dynamics of Excited-State Evolution in [Ru(bpy)3]2+
journal, January 1997

The physics of photodynamic therapy
journal, April 1986

Photodynamic Therapy with a Diode Laser for Implanted Fibrosarcoma in Mice Employing Mono-L-Aspartyl Chlorin E ₆
journal, October 1996

Photophysical and Photobiological Properties of Diporphyrin Ethers
journal, April 1991

Basic study on photochemical effect of pheophorbide a irradiated by Nd:YAG laser light [Nd: YAGレーザー光照射によるフェオフォーバイドaの光化学効果の基礎的研究]
journal, January 1986

Photodynamic therapy using pheophorbide-a and Q-switched Nd:YAG Laser on implanted human hepatocellular carcinoma
journal, October 1991

The two-photon induced fluorescence of the tumor localizing photosensitizer hematoporphyrin derivative via 1064 NM photons from a 20 NS Q-switched Nd-YAG laser
journal, January 1985

In vivo EXCITATION OF PHOTOSENSITIZERS BY INFRARED LIGHT
journal, August 1995

Spectroscopic diagnosis of esophageal cancer: New classification model, improved measurement system
journal, June 1995

Photophysical properties of the photosensitizer pheophorbide a studied at high photon flux densities
journal, February 1993

Two-Photon Excitation of 4'-Hydroxymethyl-4,5',8-Trimethylpsoralen
journal, January 1997

Two-photon molecular spectroscopy
journal, May 1974

Photoradiation Therapy. II. Cure of Animal Tumors With Hematoporphyrin and Light23
journal, July 1975

Psoralens as Photoactive Probes of Nucleic Acid Structure and Function: Organic Chemistry, Photochemistry, and Biochemistry
journal, June 1985

Pulse generation in a cw dye laser by mode−locked synchronous pumping
journal, January 1975

Two-photon excited molecular fluorescence in optically dense media
journal, November 1977

Second harmonic detection of spatially filtered two-photon excited fluorescence
journal, March 1993

Properties and Applications of Photodynamic Therapy
journal, October 1989

Experimental investigation of ultrashort pulse laser-induced breakdown thresholds in aqueous media
journal, April 1996