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Title: Method and apparatus for reducing solvent luminescence background emissions

Abstract

The detectability of luminescent molecules in solution is enhanced by reducing the background luminescence due to impurity species also present in the solution. A light source that illuminates the solution acts to photolyze the impurities so that the impurities do not luminesce in the fluorescence band of the molecule of interest. Molecules of interest may be carried through the photolysis region in the solution or may be introduced into the solution after the photolysis region.

Inventors:
 [1];  [1];  [2];  [3];  [4];  [1];  [1];  [5];  [1]
  1. Los Alamos, NM
  2. Charlottesville, VA
  3. Jemez Springs, NM
  4. Pittsburgh, PA
  5. Santa Fe, NM
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
OSTI Identifier:
871935
Patent Number(s):
5827663
Assignee:
Regents of University of California (Los Alamos, NM)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; apparatus; reducing; solvent; luminescence; background; emissions; detectability; luminescent; molecules; solution; enhanced; due; impurity; species; light; source; illuminates; photolyze; impurities; luminesce; fluorescence; band; molecule; carried; photolysis; region; introduced; photolysis region; background emissions; light source; reducing solvent; solvent luminescence; impurity species; luminescence background; luminescence due; luminescent molecules; background luminescence; /435/

Citation Formats

Affleck, Rhett L, Ambrose, W Patrick, Demas, James N, Goodwin, Peter M, Johnson, Mitchell E, Keller, Richard A, Petty, Jeffrey T, Schecker, Jay A, and Wu, Ming. Method and apparatus for reducing solvent luminescence background emissions. United States: N. p., 1998. Web.
Affleck, Rhett L, Ambrose, W Patrick, Demas, James N, Goodwin, Peter M, Johnson, Mitchell E, Keller, Richard A, Petty, Jeffrey T, Schecker, Jay A, & Wu, Ming. Method and apparatus for reducing solvent luminescence background emissions. United States.
Affleck, Rhett L, Ambrose, W Patrick, Demas, James N, Goodwin, Peter M, Johnson, Mitchell E, Keller, Richard A, Petty, Jeffrey T, Schecker, Jay A, and Wu, Ming. Thu . "Method and apparatus for reducing solvent luminescence background emissions". United States. https://www.osti.gov/servlets/purl/871935.
@article{osti_871935,
title = {Method and apparatus for reducing solvent luminescence background emissions},
author = {Affleck, Rhett L and Ambrose, W Patrick and Demas, James N and Goodwin, Peter M and Johnson, Mitchell E and Keller, Richard A and Petty, Jeffrey T and Schecker, Jay A and Wu, Ming},
abstractNote = {The detectability of luminescent molecules in solution is enhanced by reducing the background luminescence due to impurity species also present in the solution. A light source that illuminates the solution acts to photolyze the impurities so that the impurities do not luminesce in the fluorescence band of the molecule of interest. Molecules of interest may be carried through the photolysis region in the solution or may be introduced into the solution after the photolysis region.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1998},
month = {Thu Jan 01 00:00:00 EST 1998}
}

Works referenced in this record:

Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence
conference, June 1993

  • Goodwin, Peter M.; Wilkerson, Jr., Charles W.; Ambrose, W. Patrick
  • OE/LASE'93: Optics, Electro-Optics, & Laser Applications in Science& Engineering, SPIE Proceedings
  • https://doi.org/10.1117/12.146731

Detection of single fluorescent molecules
journal, November 1990


DNA sequencing by single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA
conference, June 1993

  • Goodwin, Peter M.; Schecker, Jay A.; Wilkerson, Jr., Charles W.
  • OE/LASE'93: Optics, Electro-Optics, & Laser Applications in Science& Engineering, SPIE Proceedings
  • https://doi.org/10.1117/12.146712

The Photophysical Constants of Several Fluorescent dyes Pertaining to Ultrasensitive Fluorescence Spectroscopy
journal, May 1993


Photostability studies of phycobiliprotein fluorescent labels
journal, March 1987


Single molecule detection and photochemistry on a surface using near-field optical excitation
journal, January 1994


Laser-Induced Fluorescence Detection of Rhodamine-6G at 6 × 10 −15 M
journal, June 1991