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Title: Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages

Abstract

This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods.

Inventors:
 [1];  [2]
  1. Stony Brook, NY
  2. Sound Beach, NY
Issue Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
OSTI Identifier:
871914
Patent Number(s):
5824528
Assignee:
Associated Universities, Inc. (Washington, DC)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cloning; expression; autogenes; encoding; rna; poly; erases; t7-like; bacteriophages; relates; polymerases; t7; polymerase; transcribed; promoter; recognized; encoded; achieved; reducing; activity; sufficiently; permit; host; cell; growth; controlled; combining; independent; methods; lac-repression; recombinant; operator-t7; autogene; inhibition; lysozyme; systems; producing; selected; products; described; related; materials; t7-like bacteriophages; expression systems; cell growth; related materials; rna polymerase; t7 rna; rna polymerases; host cell; producing selected; encoding rna; autogenes encoding; genes encoding; rna poly; /435/999/

Citation Formats

Studier, F William, and Dubendorff, John W. Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages. United States: N. p., 1998. Web.
Studier, F William, & Dubendorff, John W. Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages. United States.
Studier, F William, and Dubendorff, John W. Thu . "Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages". United States. https://www.osti.gov/servlets/purl/871914.
@article{osti_871914,
title = {Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages},
author = {Studier, F William and Dubendorff, John W},
abstractNote = {This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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