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Title: Recombinant cells that highly express chromosomally-integrated heterologous genes

Abstract

Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.

Inventors:
 [1];  [1];  [1]
  1. Gainesville, FL
Issue Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
OSTI Identifier:
871892
Patent Number(s):
5821093
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG05-86ER13574
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
recombinant; cells; highly; express; chromosomally-integrated; heterologous; genes; host; obtained; comprise; polypeptide-encoding; polynucleotide; segment; stably; integrated; chromosome; transcriptional; control; endogenous; promoter; mutation; effects; increased; expression; resulting; enhanced; production; polypeptide; encoded; relative; absence; achieved; retained; conditions; select; displaying; comprises; example; ethanol-production; efficient; ethanol; producer; zymomonas; mobilis; escherichia; coli; enteric; bacterial; capable; converting; wide; range; biomass-derived; sugars; efficiently; zymomonas mobilis; wide range; host cells; recombinant host; host cell; recombinant cells; segment comprises; escherichia coli; heterologous genes; stably integrated; chromosomally-integrated heterologous; express chromosomally-integrated; highly express; polypeptide-encoding polynucleotide; polynucleotide segment; /435/999/

Citation Formats

Ingram, Lonnie O, Ohta, Kazuyoshi, and Wood, Brent E. Recombinant cells that highly express chromosomally-integrated heterologous genes. United States: N. p., 1998. Web.
Ingram, Lonnie O, Ohta, Kazuyoshi, & Wood, Brent E. Recombinant cells that highly express chromosomally-integrated heterologous genes. United States.
Ingram, Lonnie O, Ohta, Kazuyoshi, and Wood, Brent E. Thu . "Recombinant cells that highly express chromosomally-integrated heterologous genes". United States. https://www.osti.gov/servlets/purl/871892.
@article{osti_871892,
title = {Recombinant cells that highly express chromosomally-integrated heterologous genes},
author = {Ingram, Lonnie O and Ohta, Kazuyoshi and Wood, Brent E},
abstractNote = {Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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Works referenced in this record:

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journal, November 1988


Purification and characterization of phosphatidylethanolamine N-methyltransferase from Zymomonas mobilis.
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Characterization of the total extractable lipids of Zymomonas mobilis var. mobilis
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Bacterial sterol surrogates. Biosynthesis of the side-chain of bacteriohopanetetrol and of a carbocyclic pseudopentose from 13C-labelled glucose in Zymomonas mobilis
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Transcriptional patterns for the thrS-infC-rplT operon of Escherichia coli
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Influence of ethanol on the hopanoid content and the fatty acid pattern in batch and continuous cultures of Zymomonas mobilis
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New method for generating deletions and gene replacements in Escherichia coli.
journal, January 1989