Method for polymer synthesis in a reaction well
Abstract
A method of synthesis for building a polymer chain, oligonucleotides in particular, by sequentially adding monomer units to at least one solid support for growing and immobilizing a polymer chain thereon in a liquid reagent solution. The method includes the step of: A) depositing a liquid reagent in a reaction well (26) in contact with at least one solid support and at least one monomer unit of the polymer chain affixed to the solid support. The well (26) includes at least one orifice (74) extending into the well (26), and is of a size and dimension to form a capillary liquid seal to retain the reagent solution in the well (26) to enable polymer chain growth on the solid support. The method further includes the step of B) expelling the reagent solution from the well (26), while retaining the polymer chain therein. This is accomplished by applying a first gas pressure to the reaction well such that a pressure differential between the first gas pressure and a second gas pressure exerted on an exit (80) of the orifice (74) exceeds a predetermined amount sufficient to overcome the capillary liquid seal and expel the reagent solution from the well (26) throughmore »
- Inventors:
-
- (San Francisco, CA)
- Issue Date:
- Research Org.:
- Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA
- OSTI Identifier:
- 871872
- Patent Number(s):
- 5814700
- Assignee:
- Board of Trustees of Leland Stanford Junior University (Stanford, CA) SNL
- DOE Contract Number:
- LBL 4437-10
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; polymer; synthesis; reaction; building; chain; oligonucleotides; particular; sequentially; adding; monomer; units; solid; support; growing; immobilizing; thereon; liquid; reagent; solution; step; depositing; 26; contact; unit; affixed; orifice; 74; extending; size; dimension; form; capillary; seal; retain; enable; growth; expelling; retaining; therein; accomplished; applying; gas; pressure; differential; exerted; exit; 80; exceeds; predetermined; amount; sufficient; overcome; expel; liquid seal; polymer synthesis; liquid reagent; solid support; pressure differential; gas pressure; amount sufficient; reagent solution; polymer chain; predetermined amount; pressure exerted; chain growth; capillary liquid; /525/530/999/
Citation Formats
Brennan, Thomas M. Method for polymer synthesis in a reaction well. United States: N. p., 1998.
Web.
Brennan, Thomas M. Method for polymer synthesis in a reaction well. United States.
Brennan, Thomas M. Thu .
"Method for polymer synthesis in a reaction well". United States. https://www.osti.gov/servlets/purl/871872.
@article{osti_871872,
title = {Method for polymer synthesis in a reaction well},
author = {Brennan, Thomas M.},
abstractNote = {A method of synthesis for building a polymer chain, oligonucleotides in particular, by sequentially adding monomer units to at least one solid support for growing and immobilizing a polymer chain thereon in a liquid reagent solution. The method includes the step of: A) depositing a liquid reagent in a reaction well (26) in contact with at least one solid support and at least one monomer unit of the polymer chain affixed to the solid support. The well (26) includes at least one orifice (74) extending into the well (26), and is of a size and dimension to form a capillary liquid seal to retain the reagent solution in the well (26) to enable polymer chain growth on the solid support. The method further includes the step of B) expelling the reagent solution from the well (26), while retaining the polymer chain therein. This is accomplished by applying a first gas pressure to the reaction well such that a pressure differential between the first gas pressure and a second gas pressure exerted on an exit (80) of the orifice (74) exceeds a predetermined amount sufficient to overcome the capillary liquid seal and expel the reagent solution from the well (26) through the orifice exit (80).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}