Title: Method for replicating an array of nucleic acid probes

Abstract

The invention relates to the replication of probe arrays and methods for replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.

Inventors:

Cantor, Charles R ^[1]; Przetakiewicz, Marek ^[1]; Smith, Cassandra L ^[1]; Sano, Takeshi ^[1]

---

+ Show Inventor Affiliations

1. Boston, MA

Issue Date:

1998-01-01

OSTI Identifier:

871784

Patent Number(s):

5795714

Assignee:

Trustees of Boston University (Boston, MA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

Show more

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/00722 - Nucleotides
B01J2219/0061 - The surface being organic
B01J2219/00612 - the surface being inorganic

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q2525/161 - incorporating target specific and non-target specific sites

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/00605 - the compounds being directly bound or immobilised to solid supports

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q2521/501 - Ligase
C12Q2565/537 - characterised by the capture oligonucleotide acting as a primer

C - CHEMISTRY C40 - COMBINATORIAL TECHNOLOGY C40B - COMBINATORIAL CHEMISTRY

C40B80/00 - Linkers or spacers specially adapted for combinatorial chemistry or libraries, e.g. traceless linkers or safety-catch linkers

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6874 - involving nucleic acid arrays, e.g. sequencing by hybridisation

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/00637 - by coating it with another layer

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6837 - using probe arrays or probe chips

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/0059 - Sequential processes

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q2525/204 - specific length of the oligonucleotides

C - CHEMISTRY C40 - COMBINATORIAL TECHNOLOGY C40B - COMBINATORIAL CHEMISTRY

C40B40/06 - Libraries containing nucleotides or polynucleotides, or derivatives thereof

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/00659 - Two-dimensional arrays
B01J2219/00644 - the porous medium being present in discrete locations, e.g. gel pads
B01J2219/00529 - DNA chips
B01J2219/00527 - Sheets
B01J19/0046 - {Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides
B01J2219/00648 - by the use of solid beads

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q2565/515 - characterised by the interaction between or sequential use of two or more arrays

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/00626 - Covalent
B01J2219/0063 - Other, e.g. van der Waals forces, hydrogen bonding

Show less

DOE Contract Number:  

PO4591610

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

method; replicating; array; nucleic; acid; probes; relates; replication; probe; arrays; methods; useful; scale; manufacture; diagnostic; aids; screen; biological; samples; specific; target; sequences; created; pcr; technology; comprise; -overhangs; biological samples; acid probes; nucleic acid; target sequence; acid probe; specific target; biological sample; /435/999/