Method for replicating an array of nucleic acid probes

Title: Method for replicating an array of nucleic acid probes

Full Record
Other Related Research

Abstract

The invention relates to the replication of probe arrays and methods for replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.

Inventors:

Cantor, Charles R. ^[1]; Przetakiewicz, Marek ^[1]; Smith, Cassandra L. ^[1]; Sano, Takeshi ^[1]

(Boston, MA)

Issue Date:: 1998-01-01

OSTI Identifier:: 871784

Patent Number(s):: 5795714

Assignee:: Trustees of Boston University (Boston, MA) OSTI

DOE Contract Number:: PO4591610

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: method; replicating; array; nucleic; acid; probes; relates; replication; probe; arrays; methods; useful; scale; manufacture; diagnostic; aids; screen; biological; samples; specific; target; sequences; created; pcr; technology; comprise; -overhangs; biological samples; acid probes; nucleic acid; target sequence; acid probe; specific target; biological sample; /435/999/

Citation Formats


                    Cantor, Charles R., Przetakiewicz, Marek, Smith, Cassandra L., and Sano, Takeshi. Method for replicating an array of nucleic acid probes.  United States: N. p., 1998. 
        Web.

Copy to clipboard


                    Cantor, Charles R., Przetakiewicz, Marek, Smith, Cassandra L., & Sano, Takeshi. Method for replicating an array of nucleic acid probes.  United States.

Copy to clipboard


                    Cantor, Charles R., Przetakiewicz, Marek, Smith, Cassandra L., and Sano, Takeshi. Thu .  
        "Method for replicating an array of nucleic acid probes".  United States.  https://www.osti.gov/servlets/purl/871784.

Copy to clipboard


                    
@article{osti_871784,

  title        = {Method for replicating an array of nucleic acid probes},

  author       = {Cantor, Charles R. and Przetakiewicz, Marek and Smith, Cassandra L. and Sano, Takeshi},

  abstractNote = {The invention relates to the replication of probe arrays and methods for replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {1}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Similar records in DOE Patents and OSTI.GOV collections:

Method for replicating an array of nucleic acid probes

Patent Cantor, C.R.; Przetakiewicz, M.; Smith, C.L.; ...

The invention relates to the replication of probe arrays and methods for replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5{prime}- and/or 3{prime}-overhangs. 16 figs.
Method for analyzing nucleic acids by means of a substrate having a microchannel structure containing immobilized nucleic acid probes

Patent Ramsey, J. Michael (Knoxville, TN); Foote, Robert S. (Oak Ridge, TN)

A method and apparatus for analyzing nucleic acids includes immobilizing nucleic probes at specific sites within a microchannel structure and moving target nucleic acids into proximity to the probes in order to allow hybridization and fluorescence detection of specific target sequences.
Full Text Available
Method for analyzing nucleic acids by means of a substrate having a microchannel structure containing immobilized nucleic acid probes

Patent Ramsey, J. Michael; Foote, Robert S.

A method and apparatus for analyzing nucleic acids includes immobilizing nucleic probes at specific sites within a microchannel structure and moving target nucleic acids into proximity to the probes in order to allow hybridization and fluorescence detection of specific target sequences.
Full Text Available
Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same

Patent Chee, Mark (Palo Alto, CA); Gingeras, Thomas R. (Santa Clara, CA); Fodor, Stephen P. A. (Palo Alto, CA); ...

The invention provides an array of oligonucleotide probes immobilized on a solid support for analysis of a target sequence from a human immunodeficiency virus. The array comprises at least four sets of oligonucleotide probes 9 to 21 nucleotides in length. A first probe set has a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus. A probe is related to its corresponding nucleotide by being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide. Thus, each probe has a position, designated an interrogation position, that is occupied by a complementarymore »« less
Full Text Available
Method for producing labeled single-stranded nucleic acid probes

Patent Dunn, John J. (Bellport, NY); Quesada, Mark A. (Middle Island, NY); Randesi, Matthew (Upton, NY)

Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment. More specifically, the method comprises providing a recombinant DNA construct comprising a DNA segment of interest inserted in a cloning vector, the cloning vector having an f1 endonuclease recognition sequence adjacent to the insertion site of the DNA segment of interest. The recombinant DNA construct is then contacted with the protein pII encoded by gene II of phage f1 thereby generating a single-stranded nick. The nicked DNA is then contacted with E. coli Exonuclease III thereby expanding the single-stranded nick into a single-stranded gap. Themore »« less
Full Text Available

Similar Records