Isolation and characterization of Agouti: a diabetes/obesity related gene

Title: Isolation and characterization of Agouti: a diabetes/obesity related gene

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Abstract

The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.

Inventors:

Woychik, Richard P. ^[1]

(Knoxville, TN)

Issue Date:: 1998-01-01

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN

OSTI Identifier:: 871757

Patent Number(s):: 5789651

Assignee:: Martin Marietta Energy Systems, Inc. (Oak Ridge, TN) ORNL

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: isolation; characterization; agouti; diabetes; obesity; related; relates; cloning; expression; analogous; genes; transformed; transfected; transgenic; mice; provides; animal; model; study; tumors; testing; potential; therapeutic; agents; oligonucleotide; probes; detection; mutations; recombinant; production; product; antibodies; diagnostic; oligonucleotide probes; therapeutic agents; recombinant production; provides oligonucleotide; transgenic mice; therapeutic agent; animal model; analogous genes; potential therapeutic; obesity related; oligonucleotide probe; /800/435/999/

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                    Woychik, Richard P. Isolation and characterization of Agouti: a diabetes/obesity related gene.  United States: N. p., 1998. 
        Web.

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                    Woychik, Richard P. Isolation and characterization of Agouti: a diabetes/obesity related gene.  United States.

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                    Woychik, Richard P. Thu .  
        "Isolation and characterization of Agouti: a diabetes/obesity related gene".  United States.  https://www.osti.gov/servlets/purl/871757.

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@article{osti_871757,

  title        = {Isolation and characterization of Agouti: a diabetes/obesity related gene},

  author       = {Woychik, Richard P.},

  abstractNote = {The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {1}

}

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Isolation and characterization of Agouti: a diabetes/obesity related gene

Patent Woychik, Richard P. (Knoxville, TN)

The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.
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The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.
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This chapter discusses the agouti gene and dominant mutations in that gene that lead to agouti-induced obesity, and recent work with transgenic mice to elucidate the role of agouti in obesity. Agouti was cloned in 1992 by the lab of Rick Woychik at Oak Ridge National Laboratory, making it the first of many recently cloned mouse obesity genes. Sequence analysis predicted that mouse agouti is a secreted protein of 131 amino acids. The mature protein has a basic central region (lys57-arg85), a proline-rich domain (pro86-pro91) and a C-terminal region (cys 92-cys 13 1) containing 10 cysteine residues which form 5more »« less
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