Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702

Title: Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702

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Abstract

Bacteria which produce large amounts of a cellulase-containing cell-free fermentate, have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase degrading bacterium ATCC 55702, which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic materials.

Inventors:

Dees, H Craig ^[1]

Lenoir City, TN

Issue Date:: 1998-01-01

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

OSTI Identifier:: 871752

Patent Number(s):: 5789227

Assignee:: Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12R - PROCESSES USING MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/2437 - {Cellulases

C - CHEMISTRY C12 - BIOCHEMISTRY C12R - PROCESSES USING MICROORGANISMS
C12R1/38 - Pseudomonas

C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS
C11D3/38645 - {containing cellulase}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/01 - Preparation of mutants without inserting foreign genetic material therein

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DOE Contract Number:: AC05-84OR21400

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: processing; cellulosic; material; cellulase-containing; cell-free; fermentate; produced; cellulase-producing; bacteria; atcc; 55702; produce; amounts; identified; original; bacterium; 55703; genetically; altered; nitrosoguanidine; mnng; treatment; enhanced; cellulase; degrading; replicate; plating; improved; characteristics; qualities; degradation; materials; cellulase-containing cell-free; improved characteristics; original bacterium; cellulosic material; enhanced cellulase; replicate plating; cellulosic materials; genetically altered; cell-free fermentate; fermentate produced; producing bacteria; /435/

Citation Formats


                    Dees, H Craig. Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702.  United States: N. p., 1998. 
        Web.

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                    Dees, H Craig. Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702.  United States.

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                    Dees, H Craig. Thu .  
        "Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702".  United States.  https://www.osti.gov/servlets/purl/871752.

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@article{osti_871752,

  title        = {Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702},

  author       = {Dees, H Craig},

  abstractNote = {Bacteria which produce large amounts of a cellulase-containing cell-free fermentate, have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase degrading bacterium ATCC 55702, which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic materials.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {1}

}

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Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702

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Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

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