Title: Dyes designed for high sensitivity detection of double-stranded DNA

Abstract

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

Inventors:

Glazer, Alexander N ^[1]; Benson, Scott C ^[2]

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1. Orinda, CA
2. Oakland, CA

Issue Date:

Thu Jan 01 00:00:00 EST 1998

Research Org.:

Univ. of California (United States)

OSTI Identifier:

871730

Patent Number(s):

5783687

Assignee:

Regents of University of California (Berkeley, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS

C - CHEMISTRY C09 - DYES C09B - ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS},

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS

C07D221/12 - Phenanthridines
C07D405/12 - linked by a chain containing hetero atoms as chain links
C07D417/06 - linked by a carbon chain containing only aliphatic carbon atoms
C07D417/14 - containing three or more hetero rings

C - CHEMISTRY C09 - DYES C09B - ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS},

C09B23/04 - one >CH- group, e.g. cyanines, isocyanines, pseudocyanines

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6816 - characterised by the detection means

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N27/44726 - {using specific dyes, markers or binding molecules}
G01N27/44747 - {Composition of gel or of carrier mixture}

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DOE Contract Number:  

FG03-91ER61125

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

dyes; designed; sensitivity; detection; double-stranded; dna; novel; fluorescent; provided; characterized; fluorophore; joined; cationic; chain; found; provide; enhancement; binding; nucleic; acid; strong; affinities; compared; polycationic; dsdna; electrophoresis; solution; substantially; sensitivities; dye; novel fluorescent; stranded dna; dyes designed; double-stranded dna; nucleic acid; fluorescent dyes; fluorescent dye; sensitivity detection; strong binding; binding affinities; polycationic chain; cationic chain; fluorophore joined; /999/435/