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Title: Surface enhanced Raman gene probe and methods thereof

Abstract

The subject invention disclosed herein is a new gene probe biosensor and methods thereof based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays.

Inventors:
 [1]
  1. (Knoxville, TN)
Issue Date:
Research Org.:
LOCKHEED MARTIN ENRGY SYST INC
OSTI Identifier:
871728
Patent Number(s):
5783389
Assignee:
Lockheed Martin Energy Research Corporation (Oak Ridge, TN) ORNL
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
surface; enhanced; raman; probe; methods; subject; disclosed; biosensor; based; scattering; sers; label; detection; ser; comprises; support; means; oligonucleotide; strand; labeled; active; substrate; disposed; probes; adsorbed; thereon; biotargets; bacterial; viral; dna; rna; pna; detected; via; hybridization; strands; complementary; supporting; fiberoptic; array; performance; multiple; assays; waveguide; microsensor; charge-coupled; devices; photodiode; arrays; sers active; probe biosensor; oligonucleotide strand; sers label; active substrate; diode arrays; photodiode array; charge-coupled device; means supporting; photodiode arrays; substrate disposed; sensor array; coupled device; raman scattering; support means; diode array; sensor comprises; enhanced raman; optic probe; surface enhanced; fiberoptic probe; waveguide microsensor; strand labeled; strands complementary; viral dna; via hybridization; probes adsorbed; microsensor array; multiple assays; oligonucleotide strands; biosensor comprises; charge-coupled devices; adsorbed thereon; probe via; nucleotide strand; nucleotide strands; fiberoptic probes; label detection; optic probes; /435/

Citation Formats

Vo-Dinh, Tuan. Surface enhanced Raman gene probe and methods thereof. United States: N. p., 1998. Web.
Vo-Dinh, Tuan. Surface enhanced Raman gene probe and methods thereof. United States.
Vo-Dinh, Tuan. Thu . "Surface enhanced Raman gene probe and methods thereof". United States. https://www.osti.gov/servlets/purl/871728.
@article{osti_871728,
title = {Surface enhanced Raman gene probe and methods thereof},
author = {Vo-Dinh, Tuan},
abstractNote = {The subject invention disclosed herein is a new gene probe biosensor and methods thereof based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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