Method for identifying and quantifying nucleic acid sequence aberrations
Abstract
A method for detecting nucleic acid sequence aberrations by detecting nucleic acid sequences having both a first and a second nucleic acid sequence type, the presence of the first and second sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. The method uses a first hybridization probe which includes a nucleic acid sequence that is complementary to a first sequence type and a first complexing agent capable of attaching to a second complexing agent and a second hybridization probe which includes a nucleic acid sequence that selectively hybridizes to the second nucleic acid sequence type over the first sequence type and includes a detectable marker for detecting the second hybridization probe.
- Inventors:
-
- San Ramon, CA
- Tracy, CA
- Walnut Creek, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 871727
- Patent Number(s):
- 5783387
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; identifying; quantifying; nucleic; acid; sequence; aberrations; detecting; sequences; type; presence; indicating; aberration; hybridization; probe; complementary; complexing; agent; capable; attaching; selectively; hybridizes; detectable; marker; sequence aberration; detecting nucleic; detectable marker; sequence aberrations; sequence type; acid sequence; nucleic acid; acid sequences; complexing agent; hybridization probe; agent capable; sequence indicating; /435/999/
Citation Formats
Lucas, Joe N, Straume, Tore, and Bogen, Kenneth T. Method for identifying and quantifying nucleic acid sequence aberrations. United States: N. p., 1998.
Web.
Lucas, Joe N, Straume, Tore, & Bogen, Kenneth T. Method for identifying and quantifying nucleic acid sequence aberrations. United States.
Lucas, Joe N, Straume, Tore, and Bogen, Kenneth T. Tue .
"Method for identifying and quantifying nucleic acid sequence aberrations". United States. https://www.osti.gov/servlets/purl/871727.
@article{osti_871727,
title = {Method for identifying and quantifying nucleic acid sequence aberrations},
author = {Lucas, Joe N and Straume, Tore and Bogen, Kenneth T},
abstractNote = {A method for detecting nucleic acid sequence aberrations by detecting nucleic acid sequences having both a first and a second nucleic acid sequence type, the presence of the first and second sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. The method uses a first hybridization probe which includes a nucleic acid sequence that is complementary to a first sequence type and a first complexing agent capable of attaching to a second complexing agent and a second hybridization probe which includes a nucleic acid sequence that selectively hybridizes to the second nucleic acid sequence type over the first sequence type and includes a detectable marker for detecting the second hybridization probe.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {7}
}
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