skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Artificial mismatch hybridization

Abstract

An improved nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. The invention has direct and advantageous application to numerous existing hybridization methods, including, applications that employ, for example, the Polymerase Chain Reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods.

Inventors:
 [1];  [1]
  1. (Madison, WI)
Issue Date:
Research Org.:
University of Wisconsin
OSTI Identifier:
871702
Patent Number(s):
5780233
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI) CHO
DOE Contract Number:  
FG02-91ER61122
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
artificial; mismatch; hybridization; improved; nucleic; acid; process; provided; employs; modified; oligonucleotide; improves; ability; discriminate; control; target; variant; containing; sequence; variation; probe; contains; relative; addition; arising; direct; advantageous; application; numerous; existing; methods; including; applications; employ; example; polymerase; chain; reaction; allele-specific; sequencing; diagnostic; hybridization methods; acid hybridization; chain reaction; nucleic acid; polymerase chain; specific nucleic; acid sequencing; acid target; sequencing method; /435/999/

Citation Formats

Guo, Zhen, and Smith, Lloyd M. Artificial mismatch hybridization. United States: N. p., 1998. Web.
Guo, Zhen, & Smith, Lloyd M. Artificial mismatch hybridization. United States.
Guo, Zhen, and Smith, Lloyd M. Thu . "Artificial mismatch hybridization". United States. https://www.osti.gov/servlets/purl/871702.
@article{osti_871702,
title = {Artificial mismatch hybridization},
author = {Guo, Zhen and Smith, Lloyd M.},
abstractNote = {An improved nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. The invention has direct and advantageous application to numerous existing hybridization methods, including, applications that employ, for example, the Polymerase Chain Reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

Patent:

Save / Share: