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Title: Artificial mismatch hybridization

Abstract

An improved nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. The invention has direct and advantageous application to numerous existing hybridization methods, including, applications that employ, for example, the Polymerase Chain Reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods.

Inventors:
 [1];  [1]
  1. Madison, WI
Issue Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
OSTI Identifier:
871702
Patent Number(s):
5780233
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-91ER61122
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
artificial; mismatch; hybridization; improved; nucleic; acid; process; provided; employs; modified; oligonucleotide; improves; ability; discriminate; control; target; variant; containing; sequence; variation; probe; contains; relative; addition; arising; direct; advantageous; application; numerous; existing; methods; including; applications; employ; example; polymerase; chain; reaction; allele-specific; sequencing; diagnostic; hybridization methods; acid hybridization; chain reaction; nucleic acid; polymerase chain; specific nucleic; acid sequencing; acid target; sequencing method; /435/999/

Citation Formats

Guo, Zhen, and Smith, Lloyd M. Artificial mismatch hybridization. United States: N. p., 1998. Web.
Guo, Zhen, & Smith, Lloyd M. Artificial mismatch hybridization. United States.
Guo, Zhen, and Smith, Lloyd M. Thu . "Artificial mismatch hybridization". United States. https://www.osti.gov/servlets/purl/871702.
@article{osti_871702,
title = {Artificial mismatch hybridization},
author = {Guo, Zhen and Smith, Lloyd M},
abstractNote = {An improved nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. The invention has direct and advantageous application to numerous existing hybridization methods, including, applications that employ, for example, the Polymerase Chain Reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

Works referenced in this record:

Predicting DNA duplex stability from the base sequence.
journal, June 1986


Synthesis, Structure, and Deoxyribonucleic Acid Sequencing with a Universal Nucleoside: 1-(2'-Deoxy-.beta.-D-ribofuranosyl)-3-nitropyrrole
journal, February 1995


Mismatches in DNA double strands: thermodynamic parameters and their correlation to repair efficiencies
journal, January 1986


Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.
journal, January 1983


5-Nitroindole as an universal base analogue
journal, January 1994


Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs
journal, January 1987


Very stable mismatch duplexes: structural and thermodynamic studies on tandem G.cntdot.A mismatches in DNA
journal, December 1992


The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin DNA
journal, January 1981


A universal nucleoside for use at ambiguous sites in DNA primers
journal, June 1994


Optical Melting of 128 Octamer DNA Duplexes
journal, April 1995