Artificial mismatch hybridization
Abstract
An improved nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. The invention has direct and advantageous application to numerous existing hybridization methods, including, applications that employ, for example, the Polymerase Chain Reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods.
- Inventors:
-
- Madison, WI
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- OSTI Identifier:
- 871702
- Patent Number(s):
- 5780233
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-91ER61122
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- artificial; mismatch; hybridization; improved; nucleic; acid; process; provided; employs; modified; oligonucleotide; improves; ability; discriminate; control; target; variant; containing; sequence; variation; probe; contains; relative; addition; arising; direct; advantageous; application; numerous; existing; methods; including; applications; employ; example; polymerase; chain; reaction; allele-specific; sequencing; diagnostic; hybridization methods; acid hybridization; chain reaction; nucleic acid; polymerase chain; specific nucleic; acid sequencing; acid target; sequencing method; /435/999/
Citation Formats
Guo, Zhen, and Smith, Lloyd M. Artificial mismatch hybridization. United States: N. p., 1998.
Web.
Guo, Zhen, & Smith, Lloyd M. Artificial mismatch hybridization. United States.
Guo, Zhen, and Smith, Lloyd M. Thu .
"Artificial mismatch hybridization". United States. https://www.osti.gov/servlets/purl/871702.
@article{osti_871702,
title = {Artificial mismatch hybridization},
author = {Guo, Zhen and Smith, Lloyd M},
abstractNote = {An improved nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. The invention has direct and advantageous application to numerous existing hybridization methods, including, applications that employ, for example, the Polymerase Chain Reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1998},
month = {Thu Jan 01 00:00:00 EST 1998}
}
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