Method of manufacturing a matrix for the detection of mismatches
Abstract
This method for preparing micromatrices consists in applying a specially-patterned intermediate layer of laser-absorbing substance on a solid support. The configuration of the sublayer fully corresponds to the topology of the manufactured matrix. The intermediate layer is further covered by a continuous layer of gel , the gel and the material of the support being transparent towards laser radiation. The gel layer is irradiated by a laser beam for a time needed to evaporate simultaneously the gel in the places immediately above the laser-absorbing sublayer and the sublayer itself. Oligonucleotides from a chosen set are then attached to the formed gel `cells`, one oligonucleotide to each cell. This method is intended for use in biotechnology, specifically for deciphering the nucleotide sequence of DNA.
- Inventors:
-
- Moscow, RU
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 871647
- Patent Number(s):
- 5770721
- Assignee:
- University of Chicago (Chicago, IL)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; manufacturing; matrix; detection; mismatches; preparing; micromatrices; consists; applying; specially-patterned; intermediate; layer; laser-absorbing; substance; solid; support; configuration; sublayer; corresponds; topology; manufactured; covered; continuous; material; transparent; towards; laser; radiation; irradiated; beam; time; evaporate; simultaneously; immediately; oligonucleotides; chosen; set; attached; formed; cells; oligonucleotide; cell; intended; biotechnology; specifically; deciphering; nucleotide; sequence; dna; solid support; nucleotide sequence; laser beam; laser radiation; intermediate layer; continuous layer; /999/216/264/422/435/
Citation Formats
Ershov, Gennady Moiseevich, and Mirzabekov, Andrei Darievich. Method of manufacturing a matrix for the detection of mismatches. United States: N. p., 1998.
Web.
Ershov, Gennady Moiseevich, & Mirzabekov, Andrei Darievich. Method of manufacturing a matrix for the detection of mismatches. United States.
Ershov, Gennady Moiseevich, and Mirzabekov, Andrei Darievich. Thu .
"Method of manufacturing a matrix for the detection of mismatches". United States. https://www.osti.gov/servlets/purl/871647.
@article{osti_871647,
title = {Method of manufacturing a matrix for the detection of mismatches},
author = {Ershov, Gennady Moiseevich and Mirzabekov, Andrei Darievich},
abstractNote = {This method for preparing micromatrices consists in applying a specially-patterned intermediate layer of laser-absorbing substance on a solid support. The configuration of the sublayer fully corresponds to the topology of the manufactured matrix. The intermediate layer is further covered by a continuous layer of gel , the gel and the material of the support being transparent towards laser radiation. The gel layer is irradiated by a laser beam for a time needed to evaporate simultaneously the gel in the places immediately above the laser-absorbing sublayer and the sublayer itself. Oligonucleotides from a chosen set are then attached to the formed gel `cells`, one oligonucleotide to each cell. This method is intended for use in biotechnology, specifically for deciphering the nucleotide sequence of DNA.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}