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Title: Method of manufacturing a matrix for the detection of mismatches

Abstract

This method for preparing micromatrices consists in applying a specially-patterned intermediate layer of laser-absorbing substance on a solid support. The configuration of the sublayer fully corresponds to the topology of the manufactured matrix. The intermediate layer is further covered by a continuous layer of gel , the gel and the material of the support being transparent towards laser radiation. The gel layer is irradiated by a laser beam for a time needed to evaporate simultaneously the gel in the places immediately above the laser-absorbing sublayer and the sublayer itself. Oligonucleotides from a chosen set are then attached to the formed gel `cells`, one oligonucleotide to each cell. This method is intended for use in biotechnology, specifically for deciphering the nucleotide sequence of DNA.

Inventors:
 [1];  [1]
  1. (Moscow, RU)
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL
OSTI Identifier:
871647
Patent Number(s):
5770721
Assignee:
University of Chicago (Chicago, IL) ANL
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; manufacturing; matrix; detection; mismatches; preparing; micromatrices; consists; applying; specially-patterned; intermediate; layer; laser-absorbing; substance; solid; support; configuration; sublayer; corresponds; topology; manufactured; covered; continuous; material; transparent; towards; laser; radiation; irradiated; beam; time; evaporate; simultaneously; immediately; oligonucleotides; chosen; set; attached; formed; cells; oligonucleotide; cell; intended; biotechnology; specifically; deciphering; nucleotide; sequence; dna; solid support; nucleotide sequence; laser beam; laser radiation; intermediate layer; continuous layer; /999/216/264/422/435/

Citation Formats

Ershov, Gennady Moiseevich, and Mirzabekov, Andrei Darievich. Method of manufacturing a matrix for the detection of mismatches. United States: N. p., 1998. Web.
Ershov, Gennady Moiseevich, & Mirzabekov, Andrei Darievich. Method of manufacturing a matrix for the detection of mismatches. United States.
Ershov, Gennady Moiseevich, and Mirzabekov, Andrei Darievich. Thu . "Method of manufacturing a matrix for the detection of mismatches". United States. https://www.osti.gov/servlets/purl/871647.
@article{osti_871647,
title = {Method of manufacturing a matrix for the detection of mismatches},
author = {Ershov, Gennady Moiseevich and Mirzabekov, Andrei Darievich},
abstractNote = {This method for preparing micromatrices consists in applying a specially-patterned intermediate layer of laser-absorbing substance on a solid support. The configuration of the sublayer fully corresponds to the topology of the manufactured matrix. The intermediate layer is further covered by a continuous layer of gel , the gel and the material of the support being transparent towards laser radiation. The gel layer is irradiated by a laser beam for a time needed to evaporate simultaneously the gel in the places immediately above the laser-absorbing sublayer and the sublayer itself. Oligonucleotides from a chosen set are then attached to the formed gel `cells`, one oligonucleotide to each cell. This method is intended for use in biotechnology, specifically for deciphering the nucleotide sequence of DNA.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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