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Title: Cytoplasmic bacteriophage display system

Abstract

Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest.

Inventors:
 [1];  [2]
  1. Stony Brook, NY
  2. Setauket, NY
Issue Date:
Research Org.:
Associated Universities, Inc., Upton, NY (United States)
OSTI Identifier:
871626
Patent Number(s):
5766905
Application Number:
08/664,449
Assignee:
Associated Universities Inc. (Washington, DC)
Patent Classifications (CPCs):
C - CHEMISTRY C40 - COMBINATORIAL TECHNOLOGY C40B - COMBINATORIAL CHEMISTRY
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cytoplasmic; bacteriophage; display; disclosed; vectors; comprising; dna; encoding; portion; structural; protein; joined; covalently; peptide; exemplified; t7; capsid; specifically; c-terminal; amino; acid; residue; n-terminal; comprises; corresponding; form; 10b; useful; copy; larger; fusion; compositions; type; described; connection; methods; producing; virus; displaying; amino acid; dna encoding; type described; display vectors; acid residue; structural protein; cytoplasmic bacteriophage; /435/424/

Citation Formats

Studier, F William, and Rosenberg, Alan H. Cytoplasmic bacteriophage display system. United States: N. p., 1998. Web.
Studier, F William, & Rosenberg, Alan H. Cytoplasmic bacteriophage display system. United States.
Studier, F William, and Rosenberg, Alan H. Tue . "Cytoplasmic bacteriophage display system". United States. https://www.osti.gov/servlets/purl/871626.
@article{osti_871626,
title = {Cytoplasmic bacteriophage display system},
author = {Studier, F William and Rosenberg, Alan H},
abstractNote = {Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {6}
}

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Works referenced in this record:

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journal, August 1990


Antibody-selectable filamentous fd phage vectors: affinity purification of target genes
journal, December 1988


Lambda foo: a lambda phage vector for the expression of foreign proteins.
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Searching for peptide ligands with an epitope library
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Expression of human factor IX and its subfragments in Escherichia coli and generation of antibodies to the subfragments
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Phage display: protein engineering by directed evolution
journal, August 1995


Display of peptides and proteins on the surface of bacteriophage lambda.
journal, February 1995