Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores

Glazer, Alexander N; Benson, Scott C

Title: Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores

Abstract

Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.

Inventors:

Glazer, Alexander N ^[1]; Benson, Scott C ^[2]

Orinda, CA
Oakland, CA

Issue Date:: 1998-01-01

Research Org.:: Univ. of California (United States)

OSTI Identifier:: 871592

Patent Number(s):: 5760201

Assignee:: Regents of University of California (Berkeley, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C09 - DYES C09B - ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS},

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS

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C - CHEMISTRY C09 - DYES C09B - ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS},
C09B23/02 - the polymethine chain containing an odd number of >CH- {or >C[alkyl]-} groups

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS
C07D417/14 - containing three or more hetero rings

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N27/44726 - {using specific dyes, markers or binding molecules}

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DOE Contract Number:: FG03-91ER61125

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: dimeric; fluorescent; energy; transfer; dyes; comprising; asymmetric; cyanine; azole-indolenine; chromophores; novel; heterodimeric; dna-staining; provided; combining; provide; strong; dna; affinity; stokes; shifts; emission; region; spectrum; particular; application; electrophoresis; labels; bound; variety; compositions; contexts; kits; individual; compounds; simultaneous; detection; moieties; particularly; single; narrow; wavelength; irradiation; source; characterized; donor; quenching; dsdna; result; optimizing; length; linking; separating; novel fluorescent; stokes shifts; single narrow; particular application; energy transfer; radiation source; narrow wavelength; transfer dyes; cyanine azole-indolenine; asymmetric cyanine; irradiation source; provided combining; simultaneous detection; strong dna; comprising asymmetric; combining asymmetric; fluorescent energy; stokes shift; azole-indolenine chromophores; azole-indolenine dyes; dyes comprising; dimeric fluorescent; dna affinity; /999/435/

Citation Formats


                    Glazer, Alexander N, and Benson, Scott C. Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores.  United States: N. p., 1998. 
        Web.

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                    Glazer, Alexander N, & Benson, Scott C. Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores.  United States.

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                    Glazer, Alexander N, and Benson, Scott C. Thu .  
        "Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores".  United States.  https://www.osti.gov/servlets/purl/871592.

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@article{osti_871592,

  title        = {Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores},

  author       = {Glazer, Alexander N and Benson, Scott C},

  abstractNote = {Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {1}

}

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Works referenced in this record:

Cyanine dye labeling reagents for sulfhydryl groups
journal, January 1989

Ernst, Lauren A.; Gupta, Ravinder K.; Mujumdar, Ratnakar B.
Cytometry, Vol. 10, Issue 1
https://doi.org/10.1002/cyto.990100103

Heterodimeric DNA-binding dyes designed for energy transfer: stability and applications of the DNA complexes
journal, January 1993

Benson, Scott C.; Mathies, Richard A.; Glazer, Alexander N.
Nucleic Acids Research, Vol. 21, Issue 24
https://doi.org/10.1093/nar/21.24.5720

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Similar Records

Title: Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores

Abstract

Citation Formats

Cyanine dye labeling reagents for sulfhydryl groups journal, January 1989

Heterodimeric DNA-binding dyes designed for energy transfer: stability and applications of the DNA complexes journal, January 1993

Cyanine dye labeling reagents for sulfhydryl groups
journal, January 1989

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journal, January 1993