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Title: Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores

Abstract

Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.

Inventors:
 [1];  [2]
  1. (Orinda, CA)
  2. (Oakland, CA)
Issue Date:
Research Org.:
University of California
OSTI Identifier:
871592
Patent Number(s):
5760201
Assignee:
Regents of University of California (Berkeley, CA) OAK
DOE Contract Number:  
FG03-91ER61125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
dimeric; fluorescent; energy; transfer; dyes; comprising; asymmetric; cyanine; azole-indolenine; chromophores; novel; heterodimeric; dna-staining; provided; combining; provide; strong; dna; affinity; stokes; shifts; emission; region; spectrum; particular; application; electrophoresis; labels; bound; variety; compositions; contexts; kits; individual; compounds; simultaneous; detection; moieties; particularly; single; narrow; wavelength; irradiation; source; characterized; donor; quenching; dsdna; result; optimizing; length; linking; separating; novel fluorescent; stokes shifts; single narrow; particular application; energy transfer; radiation source; narrow wavelength; transfer dyes; cyanine azole-indolenine; asymmetric cyanine; irradiation source; provided combining; simultaneous detection; strong dna; comprising asymmetric; combining asymmetric; fluorescent energy; stokes shift; azole-indolenine chromophores; azole-indolenine dyes; dyes comprising; dimeric fluorescent; dna affinity; /999/435/

Citation Formats

Glazer, Alexander N., and Benson, Scott C. Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores. United States: N. p., 1998. Web.
Glazer, Alexander N., & Benson, Scott C. Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores. United States.
Glazer, Alexander N., and Benson, Scott C. Thu . "Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores". United States. https://www.osti.gov/servlets/purl/871592.
@article{osti_871592,
title = {Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores},
author = {Glazer, Alexander N. and Benson, Scott C.},
abstractNote = {Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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