Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
Abstract
Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.
- Inventors:
-
- Orinda, CA
- Oakland, CA
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 871592
- Patent Number(s):
- 5760201
- Assignee:
- Regents of University of California (Berkeley, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C09 - DYES C09B - ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS},
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS
- DOE Contract Number:
- FG03-91ER61125
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- dimeric; fluorescent; energy; transfer; dyes; comprising; asymmetric; cyanine; azole-indolenine; chromophores; novel; heterodimeric; dna-staining; provided; combining; provide; strong; dna; affinity; stokes; shifts; emission; region; spectrum; particular; application; electrophoresis; labels; bound; variety; compositions; contexts; kits; individual; compounds; simultaneous; detection; moieties; particularly; single; narrow; wavelength; irradiation; source; characterized; donor; quenching; dsdna; result; optimizing; length; linking; separating; novel fluorescent; stokes shifts; single narrow; particular application; energy transfer; radiation source; narrow wavelength; transfer dyes; cyanine azole-indolenine; asymmetric cyanine; irradiation source; provided combining; simultaneous detection; strong dna; comprising asymmetric; combining asymmetric; fluorescent energy; stokes shift; azole-indolenine chromophores; azole-indolenine dyes; dyes comprising; dimeric fluorescent; dna affinity; /999/435/
Citation Formats
Glazer, Alexander N, and Benson, Scott C. Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores. United States: N. p., 1998.
Web.
Glazer, Alexander N, & Benson, Scott C. Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores. United States.
Glazer, Alexander N, and Benson, Scott C. Thu .
"Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores". United States. https://www.osti.gov/servlets/purl/871592.
@article{osti_871592,
title = {Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores},
author = {Glazer, Alexander N and Benson, Scott C},
abstractNote = {Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}