Sequence independent amplification of DNA

Bohlander, Stefan K

Title: Sequence independent amplification of DNA

Abstract

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.

Inventors:

Bohlander, Stefan K ^[1]

Chicago, IL

Issue Date:: 1998-03-24

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States)

OSTI Identifier:: 871434

Patent Number(s):: 5731171

Assignee:: Arch Development Corp. (Chicago, IL)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q2537/143 - Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
C12Q2563/107 - fluorescence
C12Q2525/155 - incorporating/generating a new priming site
C12Q1/686 - Polymerase chain reaction [PCR]

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DOE Contract Number:: FG02-86ER60408

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: sequence; independent; amplification; dna; rapid; sequence-independent; procedure; sia; minute; amounts; various; sources; amplified; requirements; priori; knowledge; characteristics; method; allows; example; microdissected; chromosomal; material; reliable; construction; quality; fluorescent; situ; hybridization; fish; probes; yacs; localize; metaphase; chromosomes; also-with; efficiency-in; interphase; nuclei; chromosomal material; situ hybridization; method allows; minute amounts; various sources; interphase nuclei; independent amplification; method allow; amplification procedure; /435/

Citation Formats


                    Bohlander, Stefan K. Sequence independent amplification of DNA.  United States: N. p., 1998. 
        Web.

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                    Bohlander, Stefan K. Sequence independent amplification of DNA.  United States.

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                    Bohlander, Stefan K. Tue .  
        "Sequence independent amplification of DNA".  United States.  https://www.osti.gov/servlets/purl/871434.

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@article{osti_871434,

  title        = {Sequence independent amplification of DNA},

  author       = {Bohlander, Stefan K},

  abstractNote = {The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {3}

}

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Works referenced in this record:

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
journal, January 1986

Mullis, K.; Faloona, F.; Scharf, S.
Cold Spring Harbor Symposia on Quantitative Biology, Vol. 51, Issue 0
https://doi.org/10.1101/SQB.1986.051.01.032

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification
journal, July 1992

Lengauer, Christoph; Green, Eric D.; Cremer, Thomas
Genomics, Vol. 13, Issue 3
https://doi.org/10.1016/0888-7543(92)90160-T

Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification
journal, March 1989

Lüdecke, Hermann-Josef; Senger, Gabriele; Claussen, Uwe
Nature, Vol. 338, Issue 6213
https://doi.org/10.1038/338348a0

Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer
journal, July 1992

Telenius, Ha˚kan; Carter, Nigel P.; Bebb, Charlotte E.
Genomics, Vol. 13, Issue 3
https://doi.org/10.1016/0888-7543(92)90147-K

Chromosomal assignment of human YAC clones by fluorescence in Situ hybridization: Use of single-yeast-colony PCR and multiple labeling
journal, September 1992

Baldini, Antonio; Ross, Mark; Nizetic, Dean
Genomics, Vol. 14, Issue 1
https://doi.org/10.1016/S0888-7543(05)80303-9

YAC mapping by FISH using Alu-PCR-generated probes
journal, July 1992

Breen, Matthew; Arveiler, Benoit; Murray, Isobel
Genomics, Vol. 13, Issue 3
https://doi.org/10.1016/0888-7543(92)90148-L

PCR amplification and analysis of yeast artificial chromosomes
journal, August 1992

Sutcliffe, James S.; Zhang, Fuping; Caskey, C. Thomas
Genomics, Vol. 13, Issue 4
https://doi.org/10.1016/0888-7543(92)90051-S

A method for the rapid sequence-independent amplification of microdissected chromosomal material
journal, August 1992

Bohlander, Stefan K.; Espinosa, Rafael; Le Beau, Michelle M.
Genomics, Vol. 13, Issue 4
https://doi.org/10.1016/0888-7543(92)90057-Y

PCR amplification of megabase DNA with tagged random primers (T-PCR)
journal, January 1993

Grothues, Dietmar; Cantor, Charles R.; Smith, Cassandra L.
Nucleic Acids Research, Vol. 21, Issue 5
https://doi.org/10.1093/nar/21.5.1321

Alu-primed polymerase chain reaction for regional assignment of 110 yeast artificial chromosome clones from the human X chromosome: identification of clones associated with a disease locus.
journal, July 1991

Nelson, D. L.; Ballabio, A.; Victoria, M. F.
Proceedings of the National Academy of Sciences, Vol. 88, Issue 14
https://doi.org/10.1073/pnas.88.14.6157

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Similar Records

Title: Sequence independent amplification of DNA

Abstract

Citation Formats

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction journal, January 1986

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification journal, July 1992

Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification journal, March 1989

Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer journal, July 1992

Chromosomal assignment of human YAC clones by fluorescence in Situ hybridization: Use of single-yeast-colony PCR and multiple labeling journal, September 1992

YAC mapping by FISH using Alu-PCR-generated probes journal, July 1992

PCR amplification and analysis of yeast artificial chromosomes journal, August 1992

A method for the rapid sequence-independent amplification of microdissected chromosomal material journal, August 1992

PCR amplification of megabase DNA with tagged random primers (T-PCR) journal, January 1993

Alu-primed polymerase chain reaction for regional assignment of 110 yeast artificial chromosome clones from the human X chromosome: identification of clones associated with a disease locus. journal, July 1991

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
journal, January 1986

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification
journal, July 1992

Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification
journal, March 1989

Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer
journal, July 1992

Chromosomal assignment of human YAC clones by fluorescence in Situ hybridization: Use of single-yeast-colony PCR and multiple labeling
journal, September 1992

YAC mapping by FISH using Alu-PCR-generated probes
journal, July 1992

PCR amplification and analysis of yeast artificial chromosomes
journal, August 1992

A method for the rapid sequence-independent amplification of microdissected chromosomal material
journal, August 1992

PCR amplification of megabase DNA with tagged random primers (T-PCR)
journal, January 1993

Alu-primed polymerase chain reaction for regional assignment of 110 yeast artificial chromosome clones from the human X chromosome: identification of clones associated with a disease locus.
journal, July 1991