Sequence independent amplification of DNA
Abstract
The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.
- Inventors:
-
- Chicago, IL
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL
- OSTI Identifier:
- 871434
- Patent Number(s):
- 5731171
- Assignee:
- Arch Development Corp. (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-86ER60408
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- sequence; independent; amplification; dna; rapid; sequence-independent; procedure; sia; minute; amounts; various; sources; amplified; requirements; priori; knowledge; characteristics; method; allows; example; microdissected; chromosomal; material; reliable; construction; quality; fluorescent; situ; hybridization; fish; probes; yacs; localize; metaphase; chromosomes; also-with; efficiency-in; interphase; nuclei; chromosomal material; situ hybridization; method allows; minute amounts; various sources; interphase nuclei; independent amplification; method allow; amplification procedure; /435/
Citation Formats
Bohlander, Stefan K. Sequence independent amplification of DNA. United States: N. p., 1998.
Web.
Bohlander, Stefan K. Sequence independent amplification of DNA. United States.
Bohlander, Stefan K. Thu .
"Sequence independent amplification of DNA". United States. https://www.osti.gov/servlets/purl/871434.
@article{osti_871434,
title = {Sequence independent amplification of DNA},
author = {Bohlander, Stefan K},
abstractNote = {The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}
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