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Title: Sequence independent amplification of DNA

Abstract

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.

Inventors:
 [1]
  1. Chicago, IL
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
871434
Patent Number(s):
5731171
Assignee:
Arch Development Corp. (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-86ER60408
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
sequence; independent; amplification; dna; rapid; sequence-independent; procedure; sia; minute; amounts; various; sources; amplified; requirements; priori; knowledge; characteristics; method; allows; example; microdissected; chromosomal; material; reliable; construction; quality; fluorescent; situ; hybridization; fish; probes; yacs; localize; metaphase; chromosomes; also-with; efficiency-in; interphase; nuclei; chromosomal material; situ hybridization; method allows; minute amounts; various sources; interphase nuclei; independent amplification; method allow; amplification procedure; /435/

Citation Formats

Bohlander, Stefan K. Sequence independent amplification of DNA. United States: N. p., 1998. Web.
Bohlander, Stefan K. Sequence independent amplification of DNA. United States.
Bohlander, Stefan K. Tue . "Sequence independent amplification of DNA". United States. https://www.osti.gov/servlets/purl/871434.
@article{osti_871434,
title = {Sequence independent amplification of DNA},
author = {Bohlander, Stefan K},
abstractNote = {The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {3}
}

Works referenced in this record:

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
journal, January 1986


Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification
journal, July 1992


Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification
journal, March 1989


Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer
journal, July 1992


YAC mapping by FISH using Alu-PCR-generated probes
journal, July 1992


PCR amplification and analysis of yeast artificial chromosomes
journal, August 1992


A method for the rapid sequence-independent amplification of microdissected chromosomal material
journal, August 1992


PCR amplification of megabase DNA with tagged random primers (T-PCR)
journal, January 1993