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Title: Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions

Abstract

A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second nucleic acid sequence type (e.g., from a second chromosome), the presence of the first and the second nucleic acid sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. In the method, immobilization of a first hybridization probe is used to isolate a first set of nucleic acids in the sample which contain the first nucleic acid sequence type. Immobilization of a second hybridization probe is then used to isolate a second set of nucleic acids from within the first set of nucleic acids which contain the second nucleic acid sequence type. The second set of nucleic acids are then detected, their presence indicating the presence of a nucleic acid sequence aberration.

Inventors:
 [1];  [2];  [3]
  1. (San Ramon, CA)
  2. (Tracy, CA)
  3. (Walnut Creek, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
871433
Patent Number(s):
5731153
Assignee:
Regents of University of California (Oakland, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
identification; random; nucleic; acid; sequence; aberrations; dual; capture; probes; hybridize; chromosome; regions; method; provided; detecting; immobilization; steps; according; aberration; detected; sequences; type; presence; indicating; hybridization; probe; isolate; set; acids; sample; contain; sequence aberration; detecting nucleic; sequence aberrations; sequence type; acid sequence; nucleic acid; acid sequences; nucleic acids; hybridization probe; sequence indicating; immobilization steps; /435/999/

Citation Formats

Lucas, Joe N., Straume, Tore, and Bogen, Kenneth T. Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions. United States: N. p., 1998. Web.
Lucas, Joe N., Straume, Tore, & Bogen, Kenneth T. Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions. United States.
Lucas, Joe N., Straume, Tore, and Bogen, Kenneth T. Thu . "Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions". United States. https://www.osti.gov/servlets/purl/871433.
@article{osti_871433,
title = {Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions},
author = {Lucas, Joe N. and Straume, Tore and Bogen, Kenneth T.},
abstractNote = {A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second nucleic acid sequence type (e.g., from a second chromosome), the presence of the first and the second nucleic acid sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. In the method, immobilization of a first hybridization probe is used to isolate a first set of nucleic acids in the sample which contain the first nucleic acid sequence type. Immobilization of a second hybridization probe is then used to isolate a second set of nucleic acids from within the first set of nucleic acids which contain the second nucleic acid sequence type. The second set of nucleic acids are then detected, their presence indicating the presence of a nucleic acid sequence aberration.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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