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Title: Recombinant Zymomonas for pentose fermentation

The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to ferment pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L-ribulokinase, and L-ribulose-5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.
Inventors:
 [1];  [2];  [3];  [4]
  1. (Golden, CO)
  2. (Lakewood, CO)
  3. (Saratoga Springs, NY)
  4. (Conifer, CO)
Issue Date:
OSTI Identifier:
871411
Assignee:
Midwest Research Institute (Kansas City, MO) NREL
Patent Number(s):
US 5726053
Contract Number:
AC36-83CH10093
Research Org:
Midwest Research Institute
Country of Publication:
United States
Language:
English
Subject:
recombinant; zymomonas; pentose; fermentation; relates; microorganisms; normally; ferment; sugar; genetically; altered; produce; ethanol; processes; utilizing; examples; mobilis; transformed; combinations; coli; genes; xylose; isomerase; xylulokinase; transaldolase; transketolase; l-arabinose; l-ribulokinase; l-ribulose-5-phosphate; 4-epimerase; expression; added; control; promoters; newly; created; useful; fermenting; pentoses; glucose; produced; hydrolysis; hemicellulose; cellulose; xylose isomerase; pentose sugar; fermentation process; zymomonas mobilis; genetically altered; produce ethanol; recombinant zymomonas; pentose fermentation; coli genes; newly created; mobilis promoters; ferment pentose; fermentation processes; added genes; fermenting pentoses; created microorganisms; l-arabinose isomerase; processes utilizing; /435/999/