skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Recombinant Zymomonas for pentose fermentation

Abstract

The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to ferment pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L-ribulokinase, and L-ribulose-5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.

Inventors:
 [1];  [2];  [3];  [4]
  1. Golden, CO
  2. Lakewood, CO
  3. Saratoga Springs, NY
  4. Conifer, CO
Issue Date:
Research Org.:
Midwest Research Institute, Kansas City, MO (United States)
OSTI Identifier:
871411
Patent Number(s):
5726053
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC36-83CH10093
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
recombinant; zymomonas; pentose; fermentation; relates; microorganisms; normally; ferment; sugar; genetically; altered; produce; ethanol; processes; utilizing; examples; mobilis; transformed; combinations; coli; genes; xylose; isomerase; xylulokinase; transaldolase; transketolase; l-arabinose; l-ribulokinase; l-ribulose-5-phosphate; 4-epimerase; expression; added; control; promoters; newly; created; useful; fermenting; pentoses; glucose; produced; hydrolysis; hemicellulose; cellulose; xylose isomerase; pentose sugar; fermentation process; zymomonas mobilis; genetically altered; produce ethanol; recombinant zymomonas; pentose fermentation; coli genes; newly created; mobilis promoters; ferment pentose; fermentation processes; added genes; fermenting pentoses; created microorganisms; l-arabinose isomerase; processes utilizing; /435/999/

Citation Formats

Picataggio, Stephen K, Zhang, Min, Eddy, Christina K, and Deanda, Kristine A. Recombinant Zymomonas for pentose fermentation. United States: N. p., 1998. Web.
Picataggio, Stephen K, Zhang, Min, Eddy, Christina K, & Deanda, Kristine A. Recombinant Zymomonas for pentose fermentation. United States.
Picataggio, Stephen K, Zhang, Min, Eddy, Christina K, and Deanda, Kristine A. Tue . "Recombinant Zymomonas for pentose fermentation". United States. https://www.osti.gov/servlets/purl/871411.
@article{osti_871411,
title = {Recombinant Zymomonas for pentose fermentation},
author = {Picataggio, Stephen K and Zhang, Min and Eddy, Christina K and Deanda, Kristine A},
abstractNote = {The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to ferment pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L-ribulokinase, and L-ribulose-5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {3}
}

Patent:

Save / Share:

Works referenced in this record:

The organization of the araBAD operon of Escherichia coli
journal, January 1986


Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains
journal, December 1992


Molecular characterization of the Zymomonas mobilis enolase (eno) gene.
journal, January 1992


Systematic sequencing of the Escherichia coli genome: analysis of the 0 – 2.4 min region
journal, January 1992


Expression of cloned Xanthomonasd-xylose catabolic genes in Zymomonas mobilis
journal, January 1988