Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase
Abstract
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .epsilon.N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed.
- Inventors:
-
- Lexington, KY
- Issue Date:
- Research Org.:
- University Of Kentucky (Lexington, KY)
- OSTI Identifier:
- 871399
- Patent Number(s):
- 5723752
- Assignee:
- University Of Kentucky (Lexington, KY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02A - TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- DOE Contract Number:
- FG05-92ER20075
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cloning; developmental; expression; pea; ribulose-1; 5-bisphosphate; carboxylase; oxygenase; subunit; n-methyltransferase; sequence; rubisco; epsilon; protein; methylase; iii; lsmt; disclosed; enzyme; catalyzes; methylation; -amine; lysine-14; addition; full-length; cdna; clone; transgenic; plants; methods; producing; inserted; dna; product; action; deleted; provided; selectively; deliver; desired; agents; plant; cdna clone; rubisco lsmt; transgenic plants; protein methylase; methylase iii; full-length cdna; pea ribulose-1; enzyme catalyzes; transgenic plant; developmental expression; /800/435/999/
Citation Formats
Houtz, Robert L. Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase. United States: N. p., 1998.
Web.
Houtz, Robert L. Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase. United States.
Houtz, Robert L. Tue .
"Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase". United States. https://www.osti.gov/servlets/purl/871399.
@article{osti_871399,
title = {Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase},
author = {Houtz, Robert L},
abstractNote = {The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .epsilon.N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 03 00:00:00 EST 1998},
month = {Tue Mar 03 00:00:00 EST 1998}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes
journal, August 1988
- Smith, C. J. S.; Watson, C. F.; Ray, J.
- Nature, Vol. 334, Issue 6184
Post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase.
journal, March 1989
- Houtz, R. L.; Stults, J. T.; Mulligan, R. M.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 6
Light-regulated methylation of chloroplast proteins.
journal, July 1987
- Black, M. T.; Meyer, D.; Widger, W. R.
- Journal of Biological Chemistry, Vol. 262, Issue 20
Isolation and characterization of a light-inducible, organ-specific gene from potato and analysis of its expression after tagging and transfer into tobacco and potato shoots
journal, October 1986
- Eckes, Peter; Rosahl, Sabine; Schell, Jeff
- Molecular and General Genetics MGG, Vol. 205, Issue 1
Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans.
journal, April 1990
- Napoli, C.; Lemieux, C.; Jorgensen, R.
- The Plant Cell
Posttranslational Modifications in the Amino- Terminal Region of the Large Subunit of Ribulose- 1,5-Bisphosphate Carboxylase/Oxygenase from Several Plant Species
journal, March 1992
- Houtz, Robert L.; Poneleit, Loelle; Jones, Samantha B.
- Plant Physiology, Vol. 98, Issue 3
Cloning and developmental expression of the sucrose-phosphate-synthase gene from spinach
journal, June 1993
- Klein, RobertR.; Crafts-Brandner, StevenJ.; Salvucci, MichaelE.
- Planta, Vol. 190, Issue 4
Partial Purification and Characterization of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit ε N -Methyltransferase
journal, November 1991
- Houtz, Robert L.; Royer, Malcolm; Salvucci, Michael E.
- Plant Physiology, Vol. 97, Issue 3
Reaction-intermediate analogue binding by ribulose bisphosphate carboxylase/oxygenase causes specific changes in proteolytic sensitivity: the amino-terminal residue of the large subunit is acetylated proline.
journal, March 1988
- Mulligan, R. M.; Houtz, R. L.; Tolbert, N. E.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 5
Protein Methylation in Pea Chloroplasts
journal, July 1990
- Niemi, Kevin J.; Adler, Julius; Selman, Bruce R.
- Plant Physiology, Vol. 93, Issue 3