Modified acyl-ACP desaturase
Abstract
Disclosed is a methods for modifying the chain length and double bond positional specificities of a soluble plant fatty acid desaturase. More specifically, the method involves modifying amino acid contact residues in the substrate binding channel of the soluble fatty acid desaturase which contact the fatty acid. Specifically disclosed is the modification of an acyl-ACP desaturase. Amino acid contact residues which lie within the substrate binding channel are identified, and subsequently replaced with different residues to effect the modification of activity.
- Inventors:
-
- Shoreham, NY
- Jarfalla, SE
- Issue Date:
- Research Org.:
- Associated Universities, Inc., Upton, NY (United States)
- OSTI Identifier:
- 871309
- Patent Number(s):
- 5705391
- Application Number:
- 08/689,823
- Assignee:
- Associated Universities, Inc. (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- modified; acyl-acp; desaturase; disclosed; methods; modifying; chain; length; double; bond; positional; specificities; soluble; plant; fatty; acid; specifically; method; involves; amino; contact; residues; substrate; binding; channel; modification; lie; identified; subsequently; replaced; effect; activity; plant fatty; acid contact; specifically disclosed; method involves; amino acid; fatty acid; chain length; contact residues; acid desaturase; acyl-acp desaturase; modified acyl-acp; method involve; modifying amino; involves modifying; soluble plant; positional specificities; bond positional; double bond; /435/999/
Citation Formats
Cahoon, Edgar B, Shanklin, John, Lindgvist, Ylva, and Schneider, Gunter. Modified acyl-ACP desaturase. United States: N. p., 1998.
Web.
Cahoon, Edgar B, Shanklin, John, Lindgvist, Ylva, & Schneider, Gunter. Modified acyl-ACP desaturase. United States.
Cahoon, Edgar B, Shanklin, John, Lindgvist, Ylva, and Schneider, Gunter. Tue .
"Modified acyl-ACP desaturase". United States. https://www.osti.gov/servlets/purl/871309.
@article{osti_871309,
title = {Modified acyl-ACP desaturase},
author = {Cahoon, Edgar B and Shanklin, John and Lindgvist, Ylva and Schneider, Gunter},
abstractNote = {Disclosed is a methods for modifying the chain length and double bond positional specificities of a soluble plant fatty acid desaturase. More specifically, the method involves modifying amino acid contact residues in the substrate binding channel of the soluble fatty acid desaturase which contact the fatty acid. Specifically disclosed is the modification of an acyl-ACP desaturase. Amino acid contact residues which lie within the substrate binding channel are identified, and subsequently replaced with different residues to effect the modification of activity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}
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