Nucleotide sequences encoding a thermostable alkaline protease
Abstract
Nucleotide sequences, derived from a thermophilic actinomycete microorganism, which encode a thermostable alkaline protease are disclosed. Also disclosed are variants of the nucleotide sequences which encode a polypeptide having thermostable alkaline proteolytic activity. Recombinant thermostable alkaline protease or recombinant polypeptide may be obtained by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant thermostable alkaline protease or recombinant polypeptide from the culture medium.
- Inventors:
-
- Ithaca, NY
- Bethesda, MD
- Issue Date:
- Research Org.:
- Cornell Univ., Ithaca, NY (United States)
- OSTI Identifier:
- 871308
- Patent Number(s):
- 5705379
- Assignee:
- Cornell Research Foundation, Inc. (Ithaca, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-84ER13233
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- nucleotide; sequences; encoding; thermostable; alkaline; protease; derived; thermophilic; actinomycete; microorganism; encode; disclosed; variants; polypeptide; proteolytic; activity; recombinant; obtained; culturing; medium; host; cell; genetically; engineered; contain; express; sequence; according; recovering; culture; sequences encoding; thermostable alkaline; culture medium; nucleotide sequences; nucleotide sequence; host cell; recombinant polypeptide; genetically engineered; alkaline protease; /435/999/
Citation Formats
Wilson, David B, and Lao, Guifang. Nucleotide sequences encoding a thermostable alkaline protease. United States: N. p., 1998.
Web.
Wilson, David B, & Lao, Guifang. Nucleotide sequences encoding a thermostable alkaline protease. United States.
Wilson, David B, and Lao, Guifang. Tue .
"Nucleotide sequences encoding a thermostable alkaline protease". United States. https://www.osti.gov/servlets/purl/871308.
@article{osti_871308,
title = {Nucleotide sequences encoding a thermostable alkaline protease},
author = {Wilson, David B and Lao, Guifang},
abstractNote = {Nucleotide sequences, derived from a thermophilic actinomycete microorganism, which encode a thermostable alkaline protease are disclosed. Also disclosed are variants of the nucleotide sequences which encode a polypeptide having thermostable alkaline proteolytic activity. Recombinant thermostable alkaline protease or recombinant polypeptide may be obtained by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant thermostable alkaline protease or recombinant polypeptide from the culture medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}
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Works referenced in this record:
Heat stable proteinase from Thermomonospora fusca: Characterization as a serine proteinase
journal, August 1990
- Kristjansson, Magnus M.; Kinsella, John E.
- International Journal of Peptide and Protein Research, Vol. 36, Issue 2