Nucleotide sequences encoding a thermostable alkaline protease

Title: Nucleotide sequences encoding a thermostable alkaline protease

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Abstract

Nucleotide sequences, derived from a thermophilic actinomycete microorganism, which encode a thermostable alkaline protease are disclosed. Also disclosed are variants of the nucleotide sequences which encode a polypeptide having thermostable alkaline proteolytic activity. Recombinant thermostable alkaline protease or recombinant polypeptide may be obtained by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant thermostable alkaline protease or recombinant polypeptide from the culture medium.

Inventors:

Wilson, David B ^[1]; Lao, Guifang ^[2]

Ithaca, NY
Bethesda, MD

Issue Date:: 1998-01-01

Research Org.:: Cornell Univ., Ithaca, NY (United States)

OSTI Identifier:: 871308

Patent Number(s):: 5705379

Assignee:: Cornell Research Foundation, Inc. (Ithaca, NY)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/52 - derived from bacteria {or Archaea}

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DOE Contract Number:: FG02-84ER13233

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: nucleotide; sequences; encoding; thermostable; alkaline; protease; derived; thermophilic; actinomycete; microorganism; encode; disclosed; variants; polypeptide; proteolytic; activity; recombinant; obtained; culturing; medium; host; cell; genetically; engineered; contain; express; sequence; according; recovering; culture; sequences encoding; thermostable alkaline; culture medium; nucleotide sequences; nucleotide sequence; host cell; recombinant polypeptide; genetically engineered; alkaline protease; /435/999/

Citation Formats


                    Wilson, David B, and Lao, Guifang. Nucleotide sequences encoding a thermostable alkaline protease.  United States: N. p., 1998. 
        Web.

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                    Wilson, David B, & Lao, Guifang. Nucleotide sequences encoding a thermostable alkaline protease.  United States.

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                    Wilson, David B, and Lao, Guifang. Thu .  
        "Nucleotide sequences encoding a thermostable alkaline protease".  United States.  https://www.osti.gov/servlets/purl/871308.

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@article{osti_871308,

  title        = {Nucleotide sequences encoding a thermostable alkaline protease},

  author       = {Wilson, David B and Lao, Guifang},

  abstractNote = {Nucleotide sequences, derived from a thermophilic actinomycete microorganism, which encode a thermostable alkaline protease are disclosed. Also disclosed are variants of the nucleotide sequences which encode a polypeptide having thermostable alkaline proteolytic activity. Recombinant thermostable alkaline protease or recombinant polypeptide may be obtained by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant thermostable alkaline protease or recombinant polypeptide from the culture medium.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {1}

}

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Nucleotide sequences encoding a thermostable alkaline protease

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Nucleotide sequences, derived from a thermophilic actinomycete microorganism, which encode a thermostable alkaline protease are disclosed. Also disclosed are variants of the nucleotide sequences which encode a polypeptide having thermostable alkaline proteolytic activity. Recombinant thermostable alkaline protease or recombinant polypeptide may be obtained by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant thermostable alkaline protease or recombinant polypeptide from the culture medium. 3 figs.
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