Title: Cellulase producing microorganism ATCC 55702

Abstract

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Inventors:

Dees, H Craig ^[1]

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1. Lenoir City, TN

Issue Date:

1997-01-01

Research Org.:

Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

OSTI Identifier:

871293

Patent Number(s):

5702940

Assignee:

Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)

Patent Classifications (CPCs):

C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS

C11D3/38645 - {containing cellulase}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/01 - Preparation of mutants without inserting foreign genetic material therein
C12N9/2437 - {Cellulases

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DOE Contract Number:  

AC05-84OR21400

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

cellulase; producing; microorganism; atcc; 55702; bacteria; produce; amounts; cellulase-containing; cell-free; fermentate; identified; original; bacterium; 55703; genetically; altered; nitrosoguanidine; mnng; treatment; enhanced; replicate; plating; improved; characteristics; qualifies; degradation; cellulosic; waste; materials; fuel; production; food; processing; textile; industrial; applications; bacterial; host; genetic; manipulations; recombinant; dna; techniques; destroy; standard; mutagenesis; cellulase producing; industrial applications; improved characteristics; original bacterium; bacterial host; enhanced cellulase; replicate plating; waste material; waste materials; recombinant dna; genetically altered; cell-free fermentate; genetic manipulations; standard mutagenesis; microorganism atcc; mutagenesis techniques; fuel production; textile processing; dna techniques; destroy genetic; improved bacterial; improved bacteria; food processing; cellulosic waste; producing bacterium; fuel product; producing microorganism; /435/