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Title: Cellulase producing microorganism ATCC 55702

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.
Inventors:
 [1]
  1. (Lenoir City, TN)
Issue Date:
OSTI Identifier:
871293
Assignee:
Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN) ORNL
Patent Number(s):
US 5702940
Contract Number:
AC05-84OR21400
Research Org:
LOCKHEED MARTIN ENRGY SYST INC
Country of Publication:
United States
Language:
English
Subject:
cellulase; producing; microorganism; atcc; 55702; bacteria; produce; amounts; cellulase-containing; cell-free; fermentate; identified; original; bacterium; 55703; genetically; altered; nitrosoguanidine; mnng; treatment; enhanced; replicate; plating; improved; characteristics; qualifies; degradation; cellulosic; waste; materials; fuel; production; food; processing; textile; industrial; applications; bacterial; host; genetic; manipulations; recombinant; dna; techniques; destroy; standard; mutagenesis; cellulase producing; industrial applications; improved characteristics; original bacterium; bacterial host; enhanced cellulase; replicate plating; waste material; waste materials; recombinant dna; genetically altered; cell-free fermentate; genetic manipulations; standard mutagenesis; microorganism atcc; mutagenesis techniques; fuel production; textile processing; dna techniques; destroy genetic; improved bacterial; improved bacteria; food processing; cellulosic waste; producing bacterium; fuel product; producing microorganism; /435/