Procedure for normalization of cDNA libraries
Abstract
This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.
- Inventors:
-
- New York, NY
- Issue Date:
- Research Org.:
- Columbia Univ., New York, NY (United States)
- OSTI Identifier:
- 871292
- Patent Number(s):
- 5702898
- Assignee:
- Trustees of Columbia University in City of New York (New York, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-91ER61233
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- procedure; normalization; cdna; libraries; provides; method; normalize; library; constructed; vector; capable; converted; single-stranded; circles; producing; complementary; nucleic; acid; molecules; comprising; converting; generating; hybridizing; step; produce; partial; duplexes; appropriate; cot; separating; unhybridized; hybridized; normalized; acid molecule; cdna libraries; produce partial; single-stranded circles; acid molecules; nucleic acid; cdna library; partial duplexes; normalized cdna; single-stranded circle; appropriate cot; complementary nucleic; library constructed; /435/999/
Citation Formats
Bonaldo, Maria DeFatima, and Soares, Marcelo Bento. Procedure for normalization of cDNA libraries. United States: N. p., 1997.
Web.
Bonaldo, Maria DeFatima, & Soares, Marcelo Bento. Procedure for normalization of cDNA libraries. United States.
Bonaldo, Maria DeFatima, and Soares, Marcelo Bento. Wed .
"Procedure for normalization of cDNA libraries". United States. https://www.osti.gov/servlets/purl/871292.
@article{osti_871292,
title = {Procedure for normalization of cDNA libraries},
author = {Bonaldo, Maria DeFatima and Soares, Marcelo Bento},
abstractNote = {This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}
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Construction of a uniform-abundance (normalized) cDNA library.
journal, March 1991
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