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Title: Procedure for normalization of cDNA libraries

This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.
Inventors:
 [1];  [1]
  1. (New York, NY)
Issue Date:
OSTI Identifier:
871292
Assignee:
Trustees of Columbia University in City of New York (New York, NY) CHO
Patent Number(s):
US 5702898
Contract Number:
FG02-91ER61233
Research Org:
Columbia University
Country of Publication:
United States
Language:
English
Subject:
procedure; normalization; cdna; libraries; provides; method; normalize; library; constructed; vector; capable; converted; single-stranded; circles; producing; complementary; nucleic; acid; molecules; comprising; converting; generating; hybridizing; step; produce; partial; duplexes; appropriate; cot; separating; unhybridized; hybridized; normalized; acid molecule; cdna libraries; produce partial; single-stranded circles; acid molecules; nucleic acid; cdna library; partial duplexes; normalized cdna; single-stranded circle; appropriate cot; complementary nucleic; library constructed; /435/999/