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Title: Site directed recombination

Abstract

Enhanced homologous recombination is obtained by employing a consensus sequence which has been found to be associated with integration of repeat sequences, such as Alu and ID. The consensus sequence or sequence having a single transition mutation determines one site of a double break which allows for high efficiency of integration at the site. By introducing single or double stranded DNA having the consensus sequence flanking region joined to a sequence of interest, one can reproducibly direct integration of the sequence of interest at one or a limited number of sites. In this way, specific sites can be identified and homologous recombination achieved at the site by employing a second flanking sequence associated with a sequence proximal to the 3'-nick.

Inventors:
 [1]
  1. Los Altos, CA
Issue Date:
Research Org.:
Genetic Information Research Institute (Palo Alto, CA)
OSTI Identifier:
871268
Patent Number(s):
5695977
Assignee:
Genetic Information Research Institute (Palo Alto, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG03-95ER62139
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
site; directed; recombination; enhanced; homologous; obtained; employing; consensus; sequence; found; associated; integration; repeat; sequences; alu; single; transition; mutation; determines; double; break; allows; efficiency; introducing; stranded; dna; flanking; region; joined; reproducibly; direct; limited; sites; specific; identified; achieved; proximal; -nick; stranded dna; double stranded; repeat sequences; repeat sequence; consensus sequence; /435/999/

Citation Formats

Jurka, Jerzy W. Site directed recombination. United States: N. p., 1997. Web.
Jurka, Jerzy W. Site directed recombination. United States.
Jurka, Jerzy W. Wed . "Site directed recombination". United States. https://www.osti.gov/servlets/purl/871268.
@article{osti_871268,
title = {Site directed recombination},
author = {Jurka, Jerzy W},
abstractNote = {Enhanced homologous recombination is obtained by employing a consensus sequence which has been found to be associated with integration of repeat sequences, such as Alu and ID. The consensus sequence or sequence having a single transition mutation determines one site of a double break which allows for high efficiency of integration at the site. By introducing single or double stranded DNA having the consensus sequence flanking region joined to a sequence of interest, one can reproducibly direct integration of the sequence of interest at one or a limited number of sites. In this way, specific sites can be identified and homologous recombination achieved at the site by employing a second flanking sequence associated with a sequence proximal to the 3'-nick.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

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Works referenced in this record:

Integration site preferences of the Alu family and similar repetitive DNA sequences
journal, January 1985


Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells.
journal, June 1994


Nonviral Retroposons: Genes, Pseudogenes, and Transposable Elements Generated by the Reverse Flow of Genetic Information
journal, June 1986


Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes.
journal, January 1995


Direct repeats flank three small nuclear RNA pseudogenes in the human genome
journal, October 1981


mRNA retroposition in human cells: processed pseudogene formation.
journal, December 1995