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Title: Site directed recombination

Enhanced homologous recombination is obtained by employing a consensus sequence which has been found to be associated with integration of repeat sequences, such as Alu and ID. The consensus sequence or sequence having a single transition mutation determines one site of a double break which allows for high efficiency of integration at the site. By introducing single or double stranded DNA having the consensus sequence flanking region joined to a sequence of interest, one can reproducibly direct integration of the sequence of interest at one or a limited number of sites. In this way, specific sites can be identified and homologous recombination achieved at the site by employing a second flanking sequence associated with a sequence proximal to the 3'-nick.
Inventors:
 [1]
  1. (Los Altos, CA)
Issue Date:
OSTI Identifier:
871268
Assignee:
Genetic Information Research Institute (Palo Alto, CA) OAK
Patent Number(s):
US 5695977
Contract Number:
FG03-95ER62139
Research Org:
Genetic Information Research Institute (Palo Alto, CA)
Country of Publication:
United States
Language:
English
Subject:
site; directed; recombination; enhanced; homologous; obtained; employing; consensus; sequence; found; associated; integration; repeat; sequences; alu; single; transition; mutation; determines; double; break; allows; efficiency; introducing; stranded; dna; flanking; region; joined; reproducibly; direct; limited; sites; specific; identified; achieved; proximal; -nick; stranded dna; double stranded; repeat sequences; repeat sequence; consensus sequence; /435/999/