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Title: Cloning and expression of the gene for bacteriophage T7 RNA polymerase

Abstract

This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.

Inventors:
 [1];  [2];  [3];  [4];  [5]
  1. (Stony Brook, NY)
  2. (Basel, CH)
  3. (Setauket, NY)
  4. (Waterloo, CA)
  5. (Bellport, NY)
Issue Date:
Research Org.:
ASSOC UNIVERSITIES INC
OSTI Identifier:
871257
Patent Number(s):
5693489
Application Number:
08/259,560
Assignee:
Associated Universities, Inc. (Washington, DC) BNL
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cloning; expression; bacteriophage; t7; rna; polymerase; application; describes; means; clone; functional; active; produced; cloned; plasmid; constructed; produce; enzyme; amounts; transcribes; dna; efficiently; highly; selective; relatively; promoter; sequence; useful; synthesizing; vivo; vitro; capable; producing; single; selectively; complex; mixture; dnas; procedure; obtain; r7; applied; t7-like; phages; clones; polymerases; specificities; bacterial; hosts; desirable; properties; high-level; synthesis; rnas; proteins; suitable; host; cells; bacteriophage t7; bacterial host; application describes; suitable host; rna polymerase; host cells; t7 rna; highly selective; rna polymerases; desirable properties; host cell; promoter specificities; promoter sequence; rna selectively; t7-like phages; single rna; active t7; bacterial hosts; produce rna; polymerase transcribes; active enzyme; obtain clones; complex mixture; transcribes dna; high-level synthesis; rna poly; /435/

Citation Formats

Studier, F. William, Davanloo, Parichehre, Rosenberg, Alan H., Moffatt, Barbara A., and Dunn, John J. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States: N. p., 1997. Web.
Studier, F. William, Davanloo, Parichehre, Rosenberg, Alan H., Moffatt, Barbara A., & Dunn, John J. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States.
Studier, F. William, Davanloo, Parichehre, Rosenberg, Alan H., Moffatt, Barbara A., and Dunn, John J. Tue . "Cloning and expression of the gene for bacteriophage T7 RNA polymerase". United States. https://www.osti.gov/servlets/purl/871257.
@article{osti_871257,
title = {Cloning and expression of the gene for bacteriophage T7 RNA polymerase},
author = {Studier, F. William and Davanloo, Parichehre and Rosenberg, Alan H. and Moffatt, Barbara A. and Dunn, John J.},
abstractNote = {This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {12}
}

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