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Title: Cloning and expression of the gene for bacteriophage T7 RNA polymerase

Abstract

This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.

Inventors:
 [1];  [2];  [3];  [4];  [5]
  1. Stony Brook, NY
  2. Basel, CH
  3. Setauket, NY
  4. Waterloo, CA
  5. Bellport, NY
Issue Date:
Research Org.:
Associated Universities, Inc., Upton, NY (United States)
OSTI Identifier:
871257
Patent Number(s):
5693489
Application Number:
08/259,560
Assignee:
Associated Universities, Inc. (Washington, DC)
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cloning; expression; bacteriophage; t7; rna; polymerase; application; describes; means; clone; functional; active; produced; cloned; plasmid; constructed; produce; enzyme; amounts; transcribes; dna; efficiently; highly; selective; relatively; promoter; sequence; useful; synthesizing; vivo; vitro; capable; producing; single; selectively; complex; mixture; dnas; procedure; obtain; r7; applied; t7-like; phages; clones; polymerases; specificities; bacterial; hosts; desirable; properties; high-level; synthesis; rnas; proteins; suitable; host; cells; bacteriophage t7; bacterial host; application describes; suitable host; rna polymerase; host cells; t7 rna; highly selective; rna polymerases; desirable properties; host cell; promoter specificities; promoter sequence; rna selectively; t7-like phages; single rna; active t7; bacterial hosts; produce rna; polymerase transcribes; active enzyme; obtain clones; complex mixture; transcribes dna; high-level synthesis; rna poly; /435/

Citation Formats

Studier, F William, Davanloo, Parichehre, Rosenberg, Alan H, Moffatt, Barbara A, and Dunn, John J. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States: N. p., 1997. Web.
Studier, F William, Davanloo, Parichehre, Rosenberg, Alan H, Moffatt, Barbara A, & Dunn, John J. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States.
Studier, F William, Davanloo, Parichehre, Rosenberg, Alan H, Moffatt, Barbara A, and Dunn, John J. Tue . "Cloning and expression of the gene for bacteriophage T7 RNA polymerase". United States. https://www.osti.gov/servlets/purl/871257.
@article{osti_871257,
title = {Cloning and expression of the gene for bacteriophage T7 RNA polymerase},
author = {Studier, F William and Davanloo, Parichehre and Rosenberg, Alan H and Moffatt, Barbara A and Dunn, John J},
abstractNote = {This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {12}
}

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