Title: High density array fabrication and readout method for a fiber optic biosensor

Abstract

The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its "sensor end" biological "binding partners" (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers aremore » then discretely addressed to detectors--such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor. « less

Inventors:

Pinkel, Daniel ^[1]; Gray, Joe ^[2]

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1. Walnut Creek, CA
2. San Francisco, CA

Issue Date:

Wed Jan 01 00:00:00 EST 1997

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

OSTI Identifier:

871246

Patent Number(s):

5690894

Assignee:

Regents of University of California (Oakland, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/00513 - Essentially linear supports
B01J2219/00524 - in the shape of fiber bundles
B01J2219/00605 - the compounds being directly bound or immobilised to solid supports
B01J2219/00612 - the surface being inorganic
B01J2219/00621 - by physical means, e.g. trenches, raised areas
B01J2219/00626 - Covalent
B01J2219/00659 - Two-dimensional arrays

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6825 - Nucleic acid detection involving sensors

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N21/6452 - {Individual samples arranged in a regular 2D-array, e.g. multiwell plates}
G01N21/648 - {using evanescent coupling or surface plasmon coupling for the excitation of fluorescence}
G01N21/7703 - {using reagent-clad optical fibres or optical waveguides
G01N2201/0833 - Fibre array at detector, resolving
G01N33/54373 - {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}

G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS

G02B6/04 - formed by bundles of fibres

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DOE Contract Number:  

AC03-76SF00098

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

density; array; fabrication; readout; method; fiber; optic; biosensor; relates; biosensors; comprising; plurality; optical; fibers; attached; sensor; biological; binding; partners; molecules; specifically; bind; form; complex; antibody-antigen; lectin-carbohydrate; nucleic; acid-nucleic; acid; biotin-avidin; etc; preferably; bears; species; partner; fabricated; providing; treated; batch; attach; bundle; uniquely; identified; combined; discretely; addressed; selected; separated; bundles; produce; capable; assaying; simultaneously; components; sample; various; transmission; detectors-such; multiplicity; sensors; signal; produced; substrate; conducted; detector; discrete; examining; transmit; unique; patterns; assisting; rapid; identification; binding partner; binding partners; sensor array; optical sensors; optical fibers; fiber optic; nucleic acid; optical fiber; optical signal; optical sensor; specifically bind; readout method; optic biosensor; density array; array fabrication; biosensors comprising; /422/65/250/435/