Title: Probes labelled with energy transfer coupled dyes

Abstract

Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.

Inventors:

Mathies, Richard A ^[1]; Glazer, Alexander ^[2]; Ju, Jingyue ^[3]

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1. El Cerrito, CA
2. Orinda, CA
3. Berkeley, CA

Issue Date:

1997-01-01

Research Org.:

Univ. of California (United States)

OSTI Identifier:

871235

Patent Number(s):

5688648

Assignee:

Regents of University of California (Oakland, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6818 - involving interaction of two or more labels, e.g. resonant energy transfer
C12Q2537/143 - Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
C12Q2565/1015 - labels being on the same oligonucleotide

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/542 - with steric inhibition or signal modification, e.g. fluorescent quenching
G01N33/582 - {with fluorescent label}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S435/81 - Packaged device or kit

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DOE Contract Number:  

FG03-91ER61125

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

probes; labelled; energy; transfer; coupled; dyes; compositions; provided; comprising; sets; fluorescent; labels; carrying; pairs; donor; acceptor; dye; molecules; designed; efficient; excitation; donors; single; wavelength; emission; wavelengths; donor-acceptor; modified; substantially; mobility; separation; conditions; varying; distance; pair; particularly; sequencing; nucleic; acids; energy transfer; nucleic acid; provided comprising; nucleic acids; dye molecules; separation conditions; fluorescent labels; single wavelength; comprising sets; fluorescent composition; acceptor pair; carrying pairs; acceptor dye; efficient excitation; labels carrying; /435/