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Title: Thermostable cellulase from a thermomonospora gene

Abstract

The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity.

Inventors:
 [1];  [1];  [1]
  1. Ithaca, NY
Issue Date:
Research Org.:
Cornell Univ., Ithaca, NY (United States)
OSTI Identifier:
871182
Patent Number(s):
5677151
Application Number:
08/265429
Assignee:
Cornell Research Foundation, Inc. (Ithaca, NY)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG02-84ER13233
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
thermostable; cellulase; thermomonospora; relates; isolated; fusca; encodes; disclosed; nucleotide; sequence; nucleic; acid; molecules; comprising; fragment; recombinantly; express; catalytically; active; polypeptide; respectively; purified; recombinant; hydrolyze; substrate; combination; cellulases; resultant; unexpected; hydrolytic; activity; acid molecule; nucleotide sequence; acid molecules; nucleic acid; catalytically active; molecules comprising; thermostable cellulase; active poly; active polypeptide; /435/424/999/

Citation Formats

Wilson, David B, Walker, Larry P, and Zhang, Sheng. Thermostable cellulase from a thermomonospora gene. United States: N. p., 1997. Web.
Wilson, David B, Walker, Larry P, & Zhang, Sheng. Thermostable cellulase from a thermomonospora gene. United States.
Wilson, David B, Walker, Larry P, and Zhang, Sheng. Wed . "Thermostable cellulase from a thermomonospora gene". United States. https://www.osti.gov/servlets/purl/871182.
@article{osti_871182,
title = {Thermostable cellulase from a thermomonospora gene},
author = {Wilson, David B and Walker, Larry P and Zhang, Sheng},
abstractNote = {The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

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Works referenced in this record:

Fragmentation of cellulose by the majorThermomonospora fusca cellulases,Trichoderma reesei CBHI, and their mixtures
journal, November 1992


Engineering cellulase mixtures by varying the mole fraction of Thermomonospora fusca E5 and E3, Trichoderma reesei CBHI, and Caldocellum saccharolyticum β-glucosidase
journal, November 1993


Synergism between pure Thermomonospora fusca and Trichoderma reesei cellulases
journal, January 1993


Activity studies of eight purified cellulases: Specificity, synergism, and binding domain effects
journal, October 1993


Biochemistry and Genetics of Actinomycete Cellulases
journal, January 1992


Hydrolysis of Cellulose by Saturating and Non–Saturating Concentrations of Cellulase: Implications for Synergism
journal, March 1988