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Title: Methods and materials relating to IMPDH and GMP production

Disclosed are purified and isolated DNA sequences encoding eukaryotic proteins possessing biological properties of inosine 5'-monophosphate dehydrogenase ("IMPDH"). Illustratively, mammalian (e.g., human) IMPDH-encoding DNA sequences are useful in transformation or transfection of host cells for the large scale recombinant production of the enzymatically active expression products and/or products (e.g., GMP) resulting from IMPDH catalyzed synthesis in cells. Vectors including IMPDH-encoding DNA sequences are useful in gene amplification procedures. Recombinant proteins and synthetic peptides provided by the invention are useful as immunological reagents and in the preparation of antibodies (including polyclonal and monoclonal antibodies) for quantitative detection of IMPDH.
 [1];  [2]
  1. (1056 Crestwood La., Bolingbrook, IL 60439)
  2. (424 Sunset Ave., LaGrange, IL 60525)
Issue Date:
OSTI Identifier:
Collart, Frank R. (1056 Crestwood La., Bolingbrook, IL 60439);Huberman, Eliezer (424 Sunset Ave., LaGrange, IL 60525) ANL
Patent Number(s):
US 5665583
Research Org:
Argonne National Laboratory (ANL), Argonne, IL
Country of Publication:
United States
methods; materials; relating; impdh; gmp; production; disclosed; purified; isolated; dna; sequences; encoding; eukaryotic; proteins; possessing; biological; properties; inosine; -monophosphate; dehydrogenase; illustratively; mammalian; human; impdh-encoding; useful; transformation; transfection; host; cells; scale; recombinant; enzymatically; active; expression; products; resulting; catalyzed; synthesis; vectors; including; amplification; procedures; synthetic; peptides; provided; immunological; reagents; preparation; antibodies; polyclonal; monoclonal; quantitative; detection; sequences encoding; dna sequences; dna sequence; isolated dna; host cells; monoclonal antibodies; host cell; recombinant production; recombinant proteins; amplification procedure; recombinant protein; /435/999/