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Title: Methods and materials relating to IMPDH and GMP production

Abstract

Disclosed are purified and isolated DNA sequences encoding eukaryotic proteins possessing biological properties of inosine 5'-monophosphate dehydrogenase ("IMPDH"). Illustratively, mammalian (e.g., human) IMPDH-encoding DNA sequences are useful in transformation or transfection of host cells for the large scale recombinant production of the enzymatically active expression products and/or products (e.g., GMP) resulting from IMPDH catalyzed synthesis in cells. Vectors including IMPDH-encoding DNA sequences are useful in gene amplification procedures. Recombinant proteins and synthetic peptides provided by the invention are useful as immunological reagents and in the preparation of antibodies (including polyclonal and monoclonal antibodies) for quantitative detection of IMPDH.

Inventors:
 [1];  [2]
  1. 1056 Crestwood La., Bolingbrook, IL 60439
  2. 424 Sunset Ave., LaGrange, IL 60525
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
871138
Patent Number(s):
5665583
Assignee:
Collart, Frank R. (1056 Crestwood La., Bolingbrook, IL 60439);Huberman, Eliezer (424 Sunset Ave., LaGrange, IL 60525)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
methods; materials; relating; impdh; gmp; production; disclosed; purified; isolated; dna; sequences; encoding; eukaryotic; proteins; possessing; biological; properties; inosine; -monophosphate; dehydrogenase; illustratively; mammalian; human; impdh-encoding; useful; transformation; transfection; host; cells; scale; recombinant; enzymatically; active; expression; products; resulting; catalyzed; synthesis; vectors; including; amplification; procedures; synthetic; peptides; provided; immunological; reagents; preparation; antibodies; polyclonal; monoclonal; quantitative; detection; sequences encoding; dna sequences; dna sequence; isolated dna; host cells; monoclonal antibodies; host cell; recombinant production; recombinant proteins; amplification procedure; recombinant protein; /435/999/

Citation Formats

Collart, Frank R, and Huberman, Eliezer. Methods and materials relating to IMPDH and GMP production. United States: N. p., 1997. Web.
Collart, Frank R, & Huberman, Eliezer. Methods and materials relating to IMPDH and GMP production. United States.
Collart, Frank R, and Huberman, Eliezer. Wed . "Methods and materials relating to IMPDH and GMP production". United States. https://www.osti.gov/servlets/purl/871138.
@article{osti_871138,
title = {Methods and materials relating to IMPDH and GMP production},
author = {Collart, Frank R and Huberman, Eliezer},
abstractNote = {Disclosed are purified and isolated DNA sequences encoding eukaryotic proteins possessing biological properties of inosine 5'-monophosphate dehydrogenase ("IMPDH"). Illustratively, mammalian (e.g., human) IMPDH-encoding DNA sequences are useful in transformation or transfection of host cells for the large scale recombinant production of the enzymatically active expression products and/or products (e.g., GMP) resulting from IMPDH catalyzed synthesis in cells. Vectors including IMPDH-encoding DNA sequences are useful in gene amplification procedures. Recombinant proteins and synthetic peptides provided by the invention are useful as immunological reagents and in the preparation of antibodies (including polyclonal and monoclonal antibodies) for quantitative detection of IMPDH.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

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Works referenced in this record:

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