Title: Methods and materials relating to IMPDH and GMP production

Abstract

Disclosed are purified and isolated DNA sequences encoding eukaryotic proteins possessing biological properties of inosine 5'-monophosphate dehydrogenase ("IMPDH"). Illustratively, mammalian (e.g., human) IMPDH-encoding DNA sequences are useful in transformation or transfection of host cells for the large scale recombinant production of the enzymatically active expression products and/or products (e.g., GMP) resulting from IMPDH catalyzed synthesis in cells. Vectors including IMPDH-encoding DNA sequences are useful in gene amplification procedures. Recombinant proteins and synthetic peptides provided by the invention are useful as immunological reagents and in the preparation of antibodies (including polyclonal and monoclonal antibodies) for quantitative detection of IMPDH.

Inventors:

Collart, Frank R ^[1]; Huberman, Eliezer ^[2]

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+ Show Inventor Affiliations

1. 1056 Crestwood La., Bolingbrook, IL 60439
2. 424 Sunset Ave., LaGrange, IL 60525

Issue Date:

1997-01-01

Research Org.:

Argonne National Laboratory (ANL), Argonne, IL (United States)

OSTI Identifier:

871138

Patent Number(s):

5665583

Assignee:

Collart, Frank R. (1056 Crestwood La., Bolingbrook, IL 60439);Huberman, Eliezer (424 Sunset Ave., LaGrange, IL 60525)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K16/40 - against enzymes

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/0006 - {acting on CH-OH groups as donors

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P19/32 - having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y101/01205 - IMP dehydrogenase

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Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

methods; materials; relating; impdh; gmp; production; disclosed; purified; isolated; dna; sequences; encoding; eukaryotic; proteins; possessing; biological; properties; inosine; -monophosphate; dehydrogenase; illustratively; mammalian; human; impdh-encoding; useful; transformation; transfection; host; cells; scale; recombinant; enzymatically; active; expression; products; resulting; catalyzed; synthesis; vectors; including; amplification; procedures; synthetic; peptides; provided; immunological; reagents; preparation; antibodies; polyclonal; monoclonal; quantitative; detection; sequences encoding; dna sequences; dna sequence; isolated dna; host cells; monoclonal antibodies; host cell; recombinant production; recombinant proteins; amplification procedure; recombinant protein; /435/999/