Large scale DNA microsequencing device
Abstract
A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.
- Inventors:
-
- Oak Ridge, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 871114
- Patent Number(s):
- 5661028
- Assignee:
- Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- scale; dna; microsequencing; device; microminiature; sequencing; apparatus; method; provide; means; simultaneously; obtaining; sequences; plural; polynucleotide; strands; comprises; microchip; channels; etched; standard; lithographic; procedures; chemical; wet; etching; reaction; separating; section; enclosing; accomplished; bonding; transparent; cover; plate; oligonucleotide; strand; chemically; affixed; alkyl; chain; subsequent; nucleotides; selected; complementary; base; pair; target; nucleotide; produce; family; labelled; channel; separated; following; separation; determined; appropriate; detection; oligonucleotide strand; alkyl chain; chemical wet; transparent cover; detection means; apparatus comprises; cover plate; method provide; wet etching; provide means; target nucleotide; apparatus comprise; complementary base; sequencing apparatus; base pair; scale dna; simultaneously obtaining; nucleotide strand; nucleotide strands; microsequencing device; microminiature sequencing; obtaining sequences; polynucleotide strands; plural polynucleotide; plural channels; dna microsequencing; lithographic procedures; /435/204/216/423/999/
Citation Formats
Foote, Robert S. Large scale DNA microsequencing device. United States: N. p., 1997.
Web.
Foote, Robert S. Large scale DNA microsequencing device. United States.
Foote, Robert S. Wed .
"Large scale DNA microsequencing device". United States. https://www.osti.gov/servlets/purl/871114.
@article{osti_871114,
title = {Large scale DNA microsequencing device},
author = {Foote, Robert S},
abstractNote = {A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1997},
month = {Wed Jan 01 00:00:00 EST 1997}
}
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Works referenced in this record:
Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip
journal, August 1993
- Harrison, D. J.; Fluri, K.; Seiler, K.
- Science, Vol. 261, Issue 5123