Fluorescent labels and their use in separations
Abstract
Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.
- Inventors:
-
- El Cerrito, CA
- Orinda, CA
- Berkeley, CA
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 871087
- Patent Number(s):
- 5654419
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG03-91ER61125
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- fluorescent; labels; separations; compositions; provided; comprising; sets; carrying; pairs; donor; acceptor; dye; molecules; designed; efficient; excitation; donors; single; wavelength; emission; wavelengths; donor-acceptor; modified; substantially; mobility; separation; conditions; varying; distance; pair; particularly; sequencing; nucleic; acids; nucleic acid; provided comprising; nucleic acids; dye molecules; separation conditions; fluorescent labels; single wavelength; comprising sets; fluorescent composition; acceptor pair; carrying pairs; acceptor dye; efficient excitation; labels carrying; /999/435/436/
Citation Formats
Mathies, Richard A, Glazer, Alexander, and Ju, Jingyue. Fluorescent labels and their use in separations. United States: N. p., 1997.
Web.
Mathies, Richard A, Glazer, Alexander, & Ju, Jingyue. Fluorescent labels and their use in separations. United States.
Mathies, Richard A, Glazer, Alexander, and Ju, Jingyue. Wed .
"Fluorescent labels and their use in separations". United States. https://www.osti.gov/servlets/purl/871087.
@article{osti_871087,
title = {Fluorescent labels and their use in separations},
author = {Mathies, Richard A and Glazer, Alexander and Ju, Jingyue},
abstractNote = {Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}
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