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Title: Fluorescent labels and their use in separations

Abstract

Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.

Inventors:
 [1];  [2];  [3]
  1. El Cerrito, CA
  2. Orinda, CA
  3. Berkeley, CA
Issue Date:
Research Org.:
Univ. of California (United States)
OSTI Identifier:
871087
Patent Number(s):
5654419
Assignee:
Regents of University of California (Oakland, CA)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG03-91ER61125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
fluorescent; labels; separations; compositions; provided; comprising; sets; carrying; pairs; donor; acceptor; dye; molecules; designed; efficient; excitation; donors; single; wavelength; emission; wavelengths; donor-acceptor; modified; substantially; mobility; separation; conditions; varying; distance; pair; particularly; sequencing; nucleic; acids; nucleic acid; provided comprising; nucleic acids; dye molecules; separation conditions; fluorescent labels; single wavelength; comprising sets; fluorescent composition; acceptor pair; carrying pairs; acceptor dye; efficient excitation; labels carrying; /999/435/436/

Citation Formats

Mathies, Richard A, Glazer, Alexander, and Ju, Jingyue. Fluorescent labels and their use in separations. United States: N. p., 1997. Web.
Mathies, Richard A, Glazer, Alexander, & Ju, Jingyue. Fluorescent labels and their use in separations. United States.
Mathies, Richard A, Glazer, Alexander, and Ju, Jingyue. Wed . "Fluorescent labels and their use in separations". United States. https://www.osti.gov/servlets/purl/871087.
@article{osti_871087,
title = {Fluorescent labels and their use in separations},
author = {Mathies, Richard A and Glazer, Alexander and Ju, Jingyue},
abstractNote = {Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

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Works referenced in this record:

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Cognitive features of continuous antigenic determinants
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Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis
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