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Title: System for sensing droplet formation time delay in a flow cytometer

Abstract

A droplet flow cytometer system which includes a system to optimize the droplet formation time delay based on conditions actually experienced includes an automatic droplet sampler which rapidly moves a plurality of containers stepwise through the droplet stream while simultaneously adjusting the droplet time delay. Through the system sampling of an actual substance to be processed can be used to minimize the effect of the substances variations or the determination of which time delay is optimal. Analysis such as cell counting and the like may be conducted manually or automatically and input to a time delay adjustment which may then act with analysis equipment to revise the time delay estimate actually applied during processing. The automatic sampler can be controlled through a microprocessor and appropriate programming to bracket an initial droplet formation time delay estimate. When maximization counts through volume, weight, or other types of analysis exists in the containers, the increment may then be reduced for a more accurate ultimate setting. This may be accomplished while actually processing the sample without interruption.

Inventors:
 [1];  [1]
  1. Seattle, WA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
871026
Patent Number(s):
5643796
Assignee:
University of Washington (Seattle, WA)
DOE Contract Number:  
FG06-93ER61662
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
sensing; droplet; formation; time; delay; flow; cytometer; optimize; based; conditions; experienced; automatic; sampler; rapidly; moves; plurality; containers; stepwise; stream; simultaneously; adjusting; sampling; substance; processed; minimize; effect; substances; variations; determination; optimal; analysis; cell; counting; conducted; manually; automatically; input; adjustment; equipment; revise; estimate; applied; processing; controlled; microprocessor; appropriate; programming; bracket; initial; maximization; counts; volume; weight; types; exists; increment; reduced; accurate; ultimate; setting; accomplished; sample; interruption; droplet formation; time delay; flow cytometer; droplet stream; formation time; delay based; /436/209/250/361/422/435/

Citation Formats

Van den Engh, Ger, and Esposito, Richard J. System for sensing droplet formation time delay in a flow cytometer. United States: N. p., 1997. Web.
Van den Engh, Ger, & Esposito, Richard J. System for sensing droplet formation time delay in a flow cytometer. United States.
Van den Engh, Ger, and Esposito, Richard J. Wed . "System for sensing droplet formation time delay in a flow cytometer". United States. https://www.osti.gov/servlets/purl/871026.
@article{osti_871026,
title = {System for sensing droplet formation time delay in a flow cytometer},
author = {Van den Engh, Ger and Esposito, Richard J},
abstractNote = {A droplet flow cytometer system which includes a system to optimize the droplet formation time delay based on conditions actually experienced includes an automatic droplet sampler which rapidly moves a plurality of containers stepwise through the droplet stream while simultaneously adjusting the droplet time delay. Through the system sampling of an actual substance to be processed can be used to minimize the effect of the substances variations or the determination of which time delay is optimal. Analysis such as cell counting and the like may be conducted manually or automatically and input to a time delay adjustment which may then act with analysis equipment to revise the time delay estimate actually applied during processing. The automatic sampler can be controlled through a microprocessor and appropriate programming to bracket an initial droplet formation time delay estimate. When maximization counts through volume, weight, or other types of analysis exists in the containers, the increment may then be reduced for a more accurate ultimate setting. This may be accomplished while actually processing the sample without interruption.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

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