System for sensing droplet formation time delay in a flow cytometer
Abstract
A droplet flow cytometer system which includes a system to optimize the droplet formation time delay based on conditions actually experienced includes an automatic droplet sampler which rapidly moves a plurality of containers stepwise through the droplet stream while simultaneously adjusting the droplet time delay. Through the system sampling of an actual substance to be processed can be used to minimize the effect of the substances variations or the determination of which time delay is optimal. Analysis such as cell counting and the like may be conducted manually or automatically and input to a time delay adjustment which may then act with analysis equipment to revise the time delay estimate actually applied during processing. The automatic sampler can be controlled through a microprocessor and appropriate programming to bracket an initial droplet formation time delay estimate. When maximization counts through volume, weight, or other types of analysis exists in the containers, the increment may then be reduced for a more accurate ultimate setting. This may be accomplished while actually processing the sample without interruption.
- Inventors:
-
- Seattle, WA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 871026
- Patent Number(s):
- 5643796
- Assignee:
- University of Washington (Seattle, WA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- DOE Contract Number:
- FG06-93ER61662
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- sensing; droplet; formation; time; delay; flow; cytometer; optimize; based; conditions; experienced; automatic; sampler; rapidly; moves; plurality; containers; stepwise; stream; simultaneously; adjusting; sampling; substance; processed; minimize; effect; substances; variations; determination; optimal; analysis; cell; counting; conducted; manually; automatically; input; adjustment; equipment; revise; estimate; applied; processing; controlled; microprocessor; appropriate; programming; bracket; initial; maximization; counts; volume; weight; types; exists; increment; reduced; accurate; ultimate; setting; accomplished; sample; interruption; droplet formation; time delay; flow cytometer; droplet stream; formation time; delay based; /436/209/250/361/422/435/
Citation Formats
Van den Engh, Ger, and Esposito, Richard J. System for sensing droplet formation time delay in a flow cytometer. United States: N. p., 1997.
Web.
Van den Engh, Ger, & Esposito, Richard J. System for sensing droplet formation time delay in a flow cytometer. United States.
Van den Engh, Ger, and Esposito, Richard J. Wed .
"System for sensing droplet formation time delay in a flow cytometer". United States. https://www.osti.gov/servlets/purl/871026.
@article{osti_871026,
title = {System for sensing droplet formation time delay in a flow cytometer},
author = {Van den Engh, Ger and Esposito, Richard J},
abstractNote = {A droplet flow cytometer system which includes a system to optimize the droplet formation time delay based on conditions actually experienced includes an automatic droplet sampler which rapidly moves a plurality of containers stepwise through the droplet stream while simultaneously adjusting the droplet time delay. Through the system sampling of an actual substance to be processed can be used to minimize the effect of the substances variations or the determination of which time delay is optimal. Analysis such as cell counting and the like may be conducted manually or automatically and input to a time delay adjustment which may then act with analysis equipment to revise the time delay estimate actually applied during processing. The automatic sampler can be controlled through a microprocessor and appropriate programming to bracket an initial droplet formation time delay estimate. When maximization counts through volume, weight, or other types of analysis exists in the containers, the increment may then be reduced for a more accurate ultimate setting. This may be accomplished while actually processing the sample without interruption.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}
Works referenced in this record:
Operation of a Flow Cytometer
book, January 1992
- Göttlinger, C.; Mechtold, B.; Radbruch, A.
- Flow Cytometry and Cell Sorting