Title: Method for synthesizing peptides with saccharide linked enzyme polymer conjugates

Abstract

A method is disclosed for synthesizing peptides using water soluble enzyme polymer conjugates. The method comprises catalyzing the peptide synthesis with enzyme which has been covalently bonded to a polymer through at least three linkers which linkers have three or more hydroxyl groups. The enzyme is conjugated at lysines or arginines.

Inventors:

Callstrom, Matthew R ^[1]; Bednarski, Mark D ^[2]; Gruber, Patrick R ^[3]

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+ Show Inventor Affiliations

1. Columbus, OH
2. Berkeley, CA
3. St. Paul, MN

Issue Date:

1997-01-01

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

OSTI Identifier:

871005

Patent Number(s):

5639633

Assignee:

Cargill, Incorporated (Minneapolis, MN)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K47/58 - obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
A61K47/6815 - {Enzymes}
A61K47/6883 - {Polymer-drug antibody conjugates, e.g. mitomycin-dextran-Ab

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

C07H13/04 - having the esterifying carboxyl radicals attached to acyclic carbon atoms
C07H15/12 - attached to a nitrogen atom of the saccharide radical
C07H5/06 - Aminosugars

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K16/1292 - {from Actinomyces
C07K16/44 - against material not provided for elsewhere {, e.g. haptens, metals, DNA, RNA, amino acids}

C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08F - MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS

C08F8/30 - Introducing nitrogen atoms or nitrogen-containing groups
C08F8/34 - Introducing sulfur atoms or sulfur-containing groups

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N11/06 - attached to the carrier via a bridging agent

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/545 - Synthetic resin
G01N33/581 - {with enzyme label

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S530/816 - Attached to the carrier via a bridging agent

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DOE Contract Number:  

AC03-76SF00098

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

method; synthesizing; peptides; saccharide; linked; enzyme; polymer; conjugates; disclosed; water; soluble; comprises; catalyzing; peptide; synthesis; covalently; bonded; linkers; hydroxyl; conjugated; lysines; arginines; water soluble; method comprises; covalently bonded; polymer conjugates; method comprise; synthesizing peptides; enzyme polymer; /435/