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Title: Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices

Abstract

The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability.

Inventors:
 [1];  [2];  [3];  [4];  [5];  [1];  [6]
  1. Newton, MA
  2. Winchester, MA
  3. Malden, MA
  4. Brookline, MA
  5. Pittsburgh, PA
  6. Quincy, MA
Issue Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
OSTI Identifier:
870968
Patent Number(s):
5633129
Assignee:
Massachusetts Institute of Technology (Cambridge, MA); Northeastern University (Boston, MA)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-86ER60448
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
electrophoretic; detection; separation; mutant; dna; replaceable; polymer; matrices; disclosure; relates; method; resolving; double-stranded; species; differing; base; pair; characterized; iso-melting; domain; unique; melting; temperature; contiguous; thermal; stability; stranded dna; double-stranded dna; thermal stability; melting temperature; disclosure relates; base pair; mutant dna; /435/204/422/436/999/

Citation Formats

Karger, Barry L, Thilly, William G, Foret, Frantisek, Khrapko, Konstaintin, Koehavong, Phouthone, Cohen, Aharon S, and Giese, Roger W. Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices. United States: N. p., 1997. Web.
Karger, Barry L, Thilly, William G, Foret, Frantisek, Khrapko, Konstaintin, Koehavong, Phouthone, Cohen, Aharon S, & Giese, Roger W. Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices. United States.
Karger, Barry L, Thilly, William G, Foret, Frantisek, Khrapko, Konstaintin, Koehavong, Phouthone, Cohen, Aharon S, and Giese, Roger W. Wed . "Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices". United States. https://www.osti.gov/servlets/purl/870968.
@article{osti_870968,
title = {Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices},
author = {Karger, Barry L and Thilly, William G and Foret, Frantisek and Khrapko, Konstaintin and Koehavong, Phouthone and Cohen, Aharon S and Giese, Roger W},
abstractNote = {The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

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Works referenced in this record:

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journal, September 1990


DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.
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Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry
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Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection
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