Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices

Karger, Barry L; Thilly, William G; Foret, Frantisek; Khrapko, Konstaintin; Koehavong, Phouthone; Cohen, Aharon S; Giese, Roger W

Advanced Search OptionsAdvanced Search queries use a traditional Term Search. For more info, see our FAQ.

All Fields:

Patent Title:

Abstract:

Assignee:

Inventor(s):

Patent Number:

Patent Classification (CPC):

All Classifications
A - human necessities
A01 - agriculture
A21 - baking
A22 - butchering
A23 - foods or foodstuffs
A24 - tobacco
A41 - wearing apparel
A42 - headwear
A43 - footwear
A44 - haberdashery
A45 - hand or travelling articles
A46 - brushware
A47 - furniture
A61 - medical or veterinary science
A62 - life-saving
A63 - sports
A99 - subject matter not otherwise provided for in this section
B - performing operations
B01 - physical or chemical processes or apparatus in general
B02 - crushing, pulverising, or disintegrating
B03 - separation of solid materials using liquids or using pneumatic tables or jigs
B04 - centrifugal apparatus or machines for carrying-out physical or chemical processes
B05 - spraying or atomising in general
B06 - generating or transmitting mechanical vibrations in general
B07 - separating solids from solids
B08 - cleaning
B09 - disposal of solid waste
B21 - mechanical metal-working without essentially removing material
B22 - casting
B23 - machine tools
B24 - grinding
B25 - hand tools
B26 - hand cutting tools
B27 - working or preserving wood or similar material
B28 - working cement, clay, or stone
B29 - working of plastics
B30 - presses
B31 - making articles of paper, cardboard or material worked in a manner analogous to paper
B32 - layered products
B33 - additive manufacturing technology
B41 - printing
B42 - bookbinding
B43 - writing or drawing implements
B44 - decorative arts
B60 - vehicles in general
B61 - railways
B62 - land vehicles for travelling otherwise than on rails
B63 - ships or other waterborne vessels
B64 - aircraft
B65 - conveying
B66 - hoisting
B67 - opening, closing {or cleaning} bottles, jars or similar containers
B68 - saddlery
B81 - microstructural technology
B82 - nanotechnology
B99 - subject matter not otherwise provided for in this section
C - chemistry
C01 - inorganic chemistry
C02 - treatment of water, waste water, sewage, or sludge
C03 - glass
C04 - cements
C05 - fertilisers
C06 - explosives
C07 - organic chemistry
C08 - organic macromolecular compounds
C09 - dyes
C10 - petroleum, gas or coke industries
C11 - animal or vegetable oils, fats, fatty substances or waxes
C12 - biochemistry
C13 - sugar industry
C14 - skins
C21 - metallurgy of iron
C22 - metallurgy
C23 - coating metallic material
C25 - electrolytic or electrophoretic processes
C30 - crystal growth
C40 - combinatorial technology
C99 - subject matter not otherwise provided for in this section
D - textiles
D01 - natural or man-made threads or fibres
D02 - yarns
D03 - weaving
D04 - braiding
D05 - sewing
D06 - treatment of textiles or the like
D07 - ropes
D10 - indexing scheme associated with sublasses of section d, relating to textiles
D21 - paper-making
D99 - subject matter not otherwise provided for in this section
E - fixed constructions
E01 - construction of roads, railways, or bridges
E02 - hydraulic engineering
E03 - water supply
E04 - building
E05 - locks
E06 - doors, windows, shutters, or roller blinds in general
E21 - earth drilling
E99 - subject matter not otherwise provided for in this section
F - mechanical engineering
F01 - machines or engines in general
F02 - combustion engines
F03 - machines or engines for liquids
F04 - positive - displacement machines for liquids
F05 - indexing schemes relating to engines or pumps in various subclasses of classes f01-f04
F15 - fluid-pressure actuators
F16 - engineering elements and units
F17 - storing or distributing gases or liquids
F21 - lighting
F22 - steam generation
F23 - combustion apparatus
F24 - heating
F25 - refrigeration or cooling
F26 - drying
F27 - furnaces
F28 - heat exchange in general
F41 - weapons
F42 - ammunition
F99 - subject matter not otherwise provided for in this section
G - physics
G01 - measuring
G02 - optics
G03 - photography
G04 - horology
G05 - controlling
G06 - computing
G07 - checking-devices
G08 - signalling
G09 - education
G10 - musical instruments
G11 - information storage
G12 - instrument details
G16 - information and communication technology [ict] specially adapted for specific application fields
G21 - nuclear physics
G99 - subject matter not otherwise provided for in this section
H - electricity
H01 - basic electric elements
H02 - generation
H03 - basic electronic circuitry
H04 - electric communication technique
H05 - electric techniques not otherwise provided for
H99 - subject matter not otherwise provided for in this section
Y - new / cross sectional technologies
Y02 - technologies or applications for mitigation or adaptation against climate change
Y04 - information or communication technologies having an impact on other technology areas
Y10 - technical subjects covered by former uspc

More Options ...

Title: Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices

Abstract

The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability.

Inventors:

Karger, Barry L ^[1]; Thilly, William G ^[2]; Foret, Frantisek ^[3]; Khrapko, Konstaintin ^[4]; Koehavong, Phouthone ^[5]; Cohen, Aharon S ^[1]; Giese, Roger W ^[6]

Newton, MA
Winchester, MA
Malden, MA
Brookline, MA
Pittsburgh, PA
Quincy, MA

Issue Date:: Wed Jan 01 00:00:00 EST 1997

Research Org.:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

OSTI Identifier:: 870968

Patent Number(s):: 5633129

Assignee:: Massachusetts Institute of Technology (Cambridge, MA); Northeastern University (Boston, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6809 - Methods for determination or identification of nucleic acids involving differential detection
C12Q1/6827 - for detection of mutation or polymorphism
C12Q2523/113 - Denaturating agents
C12Q2523/307 - Denaturation or renaturation by electric current/voltage
C12Q2525/307 - Circular oligonucleotides
C12Q2527/107 - Temperature of melting, i.e. Tm

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/81 - Packaged device or kit

Show less

DOE Contract Number:: FG02-86ER60448

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: electrophoretic; detection; separation; mutant; dna; replaceable; polymer; matrices; disclosure; relates; method; resolving; double-stranded; species; differing; base; pair; characterized; iso-melting; domain; unique; melting; temperature; contiguous; thermal; stability; stranded dna; double-stranded dna; thermal stability; melting temperature; disclosure relates; base pair; mutant dna; /435/204/422/436/999/

Citation Formats


                    Karger, Barry L, Thilly, William G, Foret, Frantisek, Khrapko, Konstaintin, Koehavong, Phouthone, Cohen, Aharon S, and Giese, Roger W. Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices.  United States: N. p., 1997. 
        Web.

Copy to clipboard


                    Karger, Barry L, Thilly, William G, Foret, Frantisek, Khrapko, Konstaintin, Koehavong, Phouthone, Cohen, Aharon S, & Giese, Roger W. Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices.  United States.

Copy to clipboard


                    Karger, Barry L, Thilly, William G, Foret, Frantisek, Khrapko, Konstaintin, Koehavong, Phouthone, Cohen, Aharon S, and Giese, Roger W. Wed .  
        "Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices".  United States.  https://www.osti.gov/servlets/purl/870968.

Copy to clipboard


                    
@article{osti_870968,

  title        = {Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices},

  author       = {Karger, Barry L and Thilly, William G and Foret, Frantisek and Khrapko, Konstaintin and Koehavong, Phouthone and Cohen, Aharon S and Giese, Roger W},

  abstractNote = {The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Wed Jan 01 00:00:00 EST 1997},

  month        = {Wed Jan 01 00:00:00 EST 1997}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis
journal, September 1990

Cohen, A. S.; Najarian, D. R.; Karger, B. L.
Journal of Chromatography A, Vol. 516, Issue 1
https://doi.org/10.1016/S0021-9673(01)90203-1

DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.
journal, March 1983

Fischer, S. G.; Lerman, L. S.
Proceedings of the National Academy of Sciences, Vol. 80, Issue 6
https://doi.org/10.1073/pnas.80.6.1579

Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry
journal, September 1988

Smith, Richard D.; Barinaga, Charles J.; Udseth, Harold R.
Analytical Chemistry, Vol. 60, Issue 18
https://doi.org/10.1021/ac00169a022

[30] Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis
book, January 1987

Lerman, Leonard S.; Silverstein, Karen
Methods in Enzymology
https://doi.org/10.1016/0076-6879(87)55032-7

Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields
journal, September 1990

Heiger, David N.; Cohen, Aharon S.; Karger, Barry L.
Journal of Chromatography A, Vol. 516, Issue 1
https://doi.org/10.1016/S0021-9673(01)90202-X

Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection
journal, January 1991

Hovig, Eivind; Smith-Sørensen, Birgitte; Brøgger, Anton
Mutation Research Letters, Vol. 262, Issue 1
https://doi.org/10.1016/0165-7992(91)90108-G

Current methods of mutation detection
journal, January 1993

Cotton, R. G. H.
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 285, Issue 1
https://doi.org/10.1016/0027-5107(93)90060-S

Capillary gel electrophoresis of biopolymers
journal, May 1993

Cohen, Aharon S.; Smisek, David L.; Keohavong, P.
TrAC Trends in Analytical Chemistry, Vol. 12, Issue 5
https://doi.org/10.1016/0165-9936(93)80020-K

Capillary zone electrophoresis with electrochemical detection
journal, July 1987

Wallingford, Ross A.; Ewing, Andrew G.
Analytical Chemistry, Vol. 59, Issue 14
https://doi.org/10.1021/ac00141a005

High-performance capillary electrophoresis of SDS-protein complexes using UV-transparent polymer networks
journal, November 1992

Ganzler, Katalin.; Greve, K. S.; Cohen, A. S.
Analytical Chemistry, Vol. 64, Issue 22
https://doi.org/10.1021/ac00046a003

p53: at the crossroads of molecular carcinogenesis and risk assessment
journal, December 1993

Harris, C.
Science, Vol. 262, Issue 5142
https://doi.org/10.1126/science.8266092

Optimization of sensitivity and separation in capillary zone electrophoresis with indirect fluorescence detection
journal, December 1988

Kuhr, Werner G.; Yeung, Edward S.
Analytical Chemistry, Vol. 60, Issue 23
https://doi.org/10.1021/ac00174a021

Rapid and quantitative detection of unique sequence donor DNA in extracts of cultured mammalian cells: An aid to chromosome mapping
journal, September 1985

Porteous, David J.
Somatic Cell and Molecular Genetics, Vol. 11, Issue 5
https://doi.org/10.1007/BF01534838

Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.
journal, December 1988

Cohen, A. S.; Najarian, D. R.; Paulus, A.
Proceedings of the National Academy of Sciences, Vol. 85, Issue 24
https://doi.org/10.1073/pnas.85.24.9660

Separation of DNA fragments by capillary electrophoresis using replaceable linear polyacrylamide matrices
journal, October 1993

Pariat, Y. F.; Berka, J.; Heiger, D. N.
Journal of Chromatography A, Vol. 652, Issue 1
https://doi.org/10.1016/0021-9673(93)80645-O

DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection
journal, October 1993

Ruiz-Martinez, Marie C.; Berka, Jan.; Belenkii, Alexei.
Analytical Chemistry, Vol. 65, Issue 20
https://doi.org/10.1021/ac00068a023

On-line capillary zone electrophoresis-ion spray tandem mass spectrometry for the determination of dynorphins
journal, December 1988

Lee, Edgar D.; Mück, Wolfgang; Henion, Jack D.
Journal of Chromatography A, Vol. 458
https://doi.org/10.1016/S0021-9673(00)90575-2

Detection of single base substitutions in total genomic DNA
journal, February 1985

Myers, Richard M.; Lumelsky, Nadya; Lerman, Leonard S.
Nature, Vol. 313, Issue 6002
https://doi.org/10.1038/313495a0

Fluorescence detection and size measurement of single DNA molecules
journal, April 1993

Castro, Alonso.; Fairfield, Frederic R.; Shera, E. Brooks.
Analytical Chemistry, Vol. 65, Issue 7
https://doi.org/10.1021/ac00055a004

Separation and detection of DNA by capillary electrophoresis
journal, December 1988

Kasper, Thomas J.; Melera, Mara; Gozel, Philippe
Journal of Chromatography A, Vol. 458
https://doi.org/10.1016/S0021-9673(00)90574-0

Capillary Electrophoresis
journal, October 1988

Gordon, M. J.; Huang, X.; Pentoney, S. L.
Science, Vol. 242, Issue 4876
https://doi.org/10.1126/science.242.4876.224

High-performance capillary electrophoresis in the biological sciences
journal, August 1989

Karger, Barry L.; Cohen, Aharon S.; Guttman, Andras
Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 492
https://doi.org/10.1016/S0378-4347(00)84480-1

Similar Records in DOE Patents and OSTI.GOV collections:

Electrophoretic device and method to separate and detect analyte ions

Patent Klavetter, Kyle C.; Siegal, Michael P.; Yelton, William G.; ...

This invention is directed to an inexpensive, miniaturized, portable, low-power device and method for electrophoretic separation and electrochemical detection of an analyte, including different isotopes of the same element. The invention replaces a conventional or microfabricated capillary electrophoresis tube with a microchip comprising an array of parallel electrophoretic separation nanotubes or aligned hollow channels fabricated in a porous substrate.
Full Text Available
Microchannel gel electrophoretic separation systems and methods for preparing and using

Patent Herr, Amy; Singh, Anup K; Throckmorton, Daniel J

A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel electrophoretic separation and quantifying analyte concentration based upon conventional polyacrylamide gel electrophoresis (PAGE). To retain biological activity of proteins and maintain intact immune complexes, native PAGE conditions were employed. Both direct (non-competitive) and competitive immunoassay formats are demonstrated in microchips for detecting toxins and biomarkers (cytokines, c-reactive protein) in bodily fluids (serum, saliva, oral fluids). Further, a description of gradient gels fabrication is included, in an effort to describe methods we have developed formore » « less
Full Text Available
Microchannel gel electrophoretic separation systems and methods for preparing and using

Patent Herr, Amy E; Singh, Anup K; Throckmorton, Daniel J

A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel electrophoretic separation and quantifying analyte concentration based upon conventional polyacrylamide gel electrophoresis (PAGE). To retain biological activity of proteins and maintain intact immune complexes, native PAGE conditions were employed. Both direct (non-competitive) and competitive immunoassay formats are demonstrated in microchips for detecting toxins and biomarkers (cytokines, c-reactive protein) in bodily fluids (serum, saliva, oral fluids). Further, a description of gradient gels fabrication is included, in an effort to describe methods we have developed formore » « less
Full Text Available
Polyacrylamide medium for the electrophoretic separation of biomolecules

Patent Madabhushi, Ramakrishna S.; Gammon, Stuart A.

A polyacryalmide medium for the electrophoretic separation of biomolecules. The polyacryalmide medium comprises high molecular weight polyacrylamides (PAAm) having a viscosity average molecular weight (M.sub.v) of about 675-725 kDa were synthesized by conventional red-ox polymerization technique. Using this separation medium, capillary electrophoresis of BigDye DNA sequencing standard was performed. A single base resolution of .about.725 bases was achieved in .about.60 minute in a non-covalently coated capillary of 50 .mu.m i.d., 40 cm effective length, and a filed of 160 V/cm at 40.degree. C. The resolution achieved with this formulation to separate DNA under identical conditions is much superior (725 basesmore » « less
Full Text Available
Combined electrophoretic-separation and electrospray method and system

Patent Smith, Richard D [Richland, WA]; Olivares, Jose A [Kennewick, WA]

A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary zone electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent atmore » « less
Full Text Available

Similar Records

Title: Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices

Abstract

Citation Formats

Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis journal, September 1990

DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. journal, March 1983

Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry journal, September 1988

[30] Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis book, January 1987

Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields journal, September 1990

Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection journal, January 1991

Current methods of mutation detection journal, January 1993

Capillary gel electrophoresis of biopolymers journal, May 1993

Capillary zone electrophoresis with electrochemical detection journal, July 1987

High-performance capillary electrophoresis of SDS-protein complexes using UV-transparent polymer networks journal, November 1992

p53: at the crossroads of molecular carcinogenesis and risk assessment journal, December 1993

Optimization of sensitivity and separation in capillary zone electrophoresis with indirect fluorescence detection journal, December 1988

Rapid and quantitative detection of unique sequence donor DNA in extracts of cultured mammalian cells: An aid to chromosome mapping journal, September 1985

Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis. journal, December 1988

Separation of DNA fragments by capillary electrophoresis using replaceable linear polyacrylamide matrices journal, October 1993

DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection journal, October 1993

On-line capillary zone electrophoresis-ion spray tandem mass spectrometry for the determination of dynorphins journal, December 1988

Detection of single base substitutions in total genomic DNA journal, February 1985

Fluorescence detection and size measurement of single DNA molecules journal, April 1993

Separation and detection of DNA by capillary electrophoresis journal, December 1988

Capillary Electrophoresis journal, October 1988

High-performance capillary electrophoresis in the biological sciences journal, August 1989

Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis
journal, September 1990

DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.
journal, March 1983

Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry
journal, September 1988

[30] Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis
book, January 1987

Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields
journal, September 1990

Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection
journal, January 1991

Current methods of mutation detection
journal, January 1993

Capillary gel electrophoresis of biopolymers
journal, May 1993

Capillary zone electrophoresis with electrochemical detection
journal, July 1987

High-performance capillary electrophoresis of SDS-protein complexes using UV-transparent polymer networks
journal, November 1992

p53: at the crossroads of molecular carcinogenesis and risk assessment
journal, December 1993

Optimization of sensitivity and separation in capillary zone electrophoresis with indirect fluorescence detection
journal, December 1988

Rapid and quantitative detection of unique sequence donor DNA in extracts of cultured mammalian cells: An aid to chromosome mapping
journal, September 1985

Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.
journal, December 1988

Separation of DNA fragments by capillary electrophoresis using replaceable linear polyacrylamide matrices
journal, October 1993

DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection
journal, October 1993

On-line capillary zone electrophoresis-ion spray tandem mass spectrometry for the determination of dynorphins
journal, December 1988

Detection of single base substitutions in total genomic DNA
journal, February 1985

Fluorescence detection and size measurement of single DNA molecules
journal, April 1993

Separation and detection of DNA by capillary electrophoresis
journal, December 1988

Capillary Electrophoresis
journal, October 1988

High-performance capillary electrophoresis in the biological sciences
journal, August 1989