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Title: Method and apparatus for detection of fluorescently labeled materials

Abstract

Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.

Inventors:
 [1];  [2]
  1. Mt. View, CA
  2. Los Altos, CA
Issue Date:
Research Org.:
Affymax Research Institute
OSTI Identifier:
870962
Patent Number(s):
5631734
Assignee:
Affymetrix, Inc. (Santa Clara, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
DOE Contract Number:  
FG03-92ER81275
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; apparatus; detection; fluorescently; labeled; materials; marked; targets; bind; substrate; 230; synthesized; polymer; sequences; locations; detected; exposing; selected; regions; light; source; 100; detecting; photons; fluoresced; therefrom; repeating; steps; exposure; completely; examined; resulting; data; determine; binding; affinity; specific; light source; selected regions; resulting data; fluorescently labeled; selected region; specific polymer; /356/250/

Citation Formats

Stern, David, and Fiekowsky, Peter. Method and apparatus for detection of fluorescently labeled materials. United States: N. p., 1997. Web.
Stern, David, & Fiekowsky, Peter. Method and apparatus for detection of fluorescently labeled materials. United States.
Stern, David, and Fiekowsky, Peter. Wed . "Method and apparatus for detection of fluorescently labeled materials". United States. https://www.osti.gov/servlets/purl/870962.
@article{osti_870962,
title = {Method and apparatus for detection of fluorescently labeled materials},
author = {Stern, David and Fiekowsky, Peter},
abstractNote = {Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

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Works referenced in this record:

Features and Applications of the Laser Scanning Microscope
journal, November 1990


Confocal compact scanning optical microscope based on compact disc technology
journal, January 1991


Development of microspot multi-analyte ratiometric immunoassay using dual fluorescent-labelled antibodies
journal, January 1989


An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy.
journal, July 1987


Multianalyte microspot immunoassay--microanalytical "compact disk" of the future
journal, November 1991


Design and use of a computer controlled confocal microscope for biological applications: Confocal microscope for biological applications
journal, January 1985