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Title: Luminescent lanthanide chelates and methods of use

Abstract

The invention provides lanthanide chelates capable of intense luminescence. The celates comprise a lanthanide chelator covalently joined to a coumarin-like or quinolone-like sensitizer. Exemplary sensitzers include 2- or 4-quinolones, 2- or 4-coumarins, or derivatives thereof e.g. carbostyril 124 (7-amino-4-methyl-2-quinolone), coumarin 120 (7-amino-4-methyl-2-coumarin), coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminomethyltrimethylpsoralen, etc. The chelates form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. The chelates may be coupled to a wide variety of compounds to create specific labels, probes, diagnostic and/or therapeutic reagents, etc. The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. The methods provide useful information about the structure, conformation, relative location and/or interactions of macromolecules.

Inventors:
 [1];  [1]
+ Show Inventor Affiliations
  1. Berkeley, CA
Issue Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
OSTI Identifier:
870909
Patent Number(s):
5622821
Assignee:
Regents of University of California (Oakland, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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DOE Contract Number:  
AC04-76
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
luminescent; lanthanide; chelates; methods; provides; capable; intense; luminescence; celates; comprise; chelator; covalently; joined; coumarin-like; quinolone-like; sensitizer; exemplary; sensitzers; 2-; 4-quinolones; 4-coumarins; derivatives; carbostyril; 124; 7-amino-4-methyl-2-quinolone; coumarin; 120; 7-amino-4-methyl-2-coumarin; 7-amino-4-; trifluoromethyl; -2-coumarin; aminomethyltrimethylpsoralen; etc; form; affinity; complexes; lanthanides; terbium; europium; dtpa; coupled; wide; variety; compounds; create; specific; labels; probes; diagnostic; therapeutic; reagents; particular; resonance; energy; transfer; chelate-lanthanide; agent; fluorescent; non-metal; based; acceptor; provide; useful; information; structure; conformation; relative; location; interactions; macromolecules; resonance energy; energy transfer; wide variety; methods provide; useful information; relative location; provide useful; metal base; lanthanide chelates; provides lanthanide; quinolone-like sensitizer; celates comprise; chelator covalently; chelates form; chelates capable; metal based; intense luminescence; lanthanide chelator; luminescent lanthanide; covalently joined; /435/436/530/999/

Citation Formats

Selvin, Paul R, and Hearst, John. Luminescent lanthanide chelates and methods of use. United States: N. p., 1997. Web.
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Selvin, Paul R, & Hearst, John. Luminescent lanthanide chelates and methods of use. United States.
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Selvin, Paul R, and Hearst, John. Wed . "Luminescent lanthanide chelates and methods of use". United States. https://www.osti.gov/servlets/purl/870909.
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@article{osti_870909,
title = {Luminescent lanthanide chelates and methods of use},
author = {Selvin, Paul R and Hearst, John},
abstractNote = {The invention provides lanthanide chelates capable of intense luminescence. The celates comprise a lanthanide chelator covalently joined to a coumarin-like or quinolone-like sensitizer. Exemplary sensitzers include 2- or 4-quinolones, 2- or 4-coumarins, or derivatives thereof e.g. carbostyril 124 (7-amino-4-methyl-2-quinolone), coumarin 120 (7-amino-4-methyl-2-coumarin), coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminomethyltrimethylpsoralen, etc. The chelates form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. The chelates may be coupled to a wide variety of compounds to create specific labels, probes, diagnostic and/or therapeutic reagents, etc. The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. The methods provide useful information about the structure, conformation, relative location and/or interactions of macromolecules.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}
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Works referenced in this record:

Fluorescent terbium chelates derived from diethylenetriaminepentaacetic acid and heterocyclic compounds
journal, January 1989

Rare earth cryptates and homogeneous fluoroimmunoassays with human sera
journal, September 1993

[13] Fluorescence resonance energy transfer
book, January 1995

Synthesis of a highly luminescent terbium chelate and its application to actin
journal, September 1992

Time-resolved fluorescence of a new europium-chelate complex: demonstration of highly sensitive detection of protein and DNA samples
journal, November 1993

Terbium chelate for use as a label in fluorescent immunoassays
journal, January 1984

A Study of Sensitized Lanthanide Luminescence in an Engineered Calcium-Binding Protein
journal, April 1993

Time-resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization
journal, January 1992

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