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Title: Luminescent lanthanide chelates and methods of use

The invention provides lanthanide chelates capable of intense luminescence. The celates comprise a lanthanide chelator covalently joined to a coumarin-like or quinolone-like sensitizer. Exemplary sensitzers include 2- or 4-quinolones, 2- or 4-coumarins, or derivatives thereof e.g. carbostyril 124 (7-amino-4-methyl-2-quinolone), coumarin 120 (7-amino-4-methyl-2-coumarin), coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminomethyltrimethylpsoralen, etc. The chelates form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. The chelates may be coupled to a wide variety of compounds to create specific labels, probes, diagnostic and/or therapeutic reagents, etc. The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. The methods provide useful information about the structure, conformation, relative location and/or interactions of macromolecules.
Inventors:
 [1];  [1]
  1. (Berkeley, CA)
Issue Date:
OSTI Identifier:
870909
Assignee:
Regents of University of California (Oakland, CA) SNL
Patent Number(s):
US 5622821
Contract Number:
AC04-76
Research Org:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA
Country of Publication:
United States
Language:
English
Subject:
luminescent; lanthanide; chelates; methods; provides; capable; intense; luminescence; celates; comprise; chelator; covalently; joined; coumarin-like; quinolone-like; sensitizer; exemplary; sensitzers; 2-; 4-quinolones; 4-coumarins; derivatives; carbostyril; 124; 7-amino-4-methyl-2-quinolone; coumarin; 120; 7-amino-4-methyl-2-coumarin; 7-amino-4-; trifluoromethyl; -2-coumarin; aminomethyltrimethylpsoralen; etc; form; affinity; complexes; lanthanides; terbium; europium; dtpa; coupled; wide; variety; compounds; create; specific; labels; probes; diagnostic; therapeutic; reagents; particular; resonance; energy; transfer; chelate-lanthanide; agent; fluorescent; non-metal; based; acceptor; provide; useful; information; structure; conformation; relative; location; interactions; macromolecules; resonance energy; energy transfer; wide variety; methods provide; useful information; relative location; provide useful; metal base; lanthanide chelates; provides lanthanide; quinolone-like sensitizer; celates comprise; chelator covalently; chelates form; chelates capable; metal based; intense luminescence; lanthanide chelator; luminescent lanthanide; covalently joined; /435/436/530/999/

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