Detection and isolation of nucleic acid sequences using competitive hybridization probes

Lucas, Joe N; Straume, Tore; Bogen, Kenneth T

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Title: Detection and isolation of nucleic acid sequences using competitive hybridization probes

Abstract

A method for detecting a target nucleic acid sequence in a sample is provided using hybridization probes which competitively hybridize to a target nucleic acid. According to the method, a target nucleic acid sequence is hybridized to first and second hybridization probes which are complementary to overlapping portions of the target nucleic acid sequence, the first hybridization probe including a first complexing agent capable of forming a binding pair with a second complexing agent and the second hybridization probe including a detectable marker. The first complexing agent attached to the first hybridization probe is contacted with a second complexing agent, the second complexing agent being attached to a solid support such that when the first and second complexing agents are attached, target nucleic acid sequences hybridized to the first hybridization probe become immobilized on to the solid support. The immobilized target nucleic acids are then separated and detected by detecting the detectable marker attached to the second hybridization probe. A kit for performing the method is also provided.

Inventors:

Lucas, Joe N ^[1]; Straume, Tore ^[2]; Bogen, Kenneth T ^[3]

San Ramon, CA
Tracy, CA
Walnut Creek, CA

Issue Date:: 1997-01-01

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

OSTI Identifier:: 870890

Patent Number(s):: 5616465

Assignee:: Regents of University of California (Oakland, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6813 - Hybridisation assays
C12Q1/6823 - Release of bound markers

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DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: detection; isolation; nucleic; acid; sequences; competitive; hybridization; probes; method; detecting; target; sequence; sample; provided; competitively; hybridize; according; hybridized; complementary; overlapping; portions; probe; including; complexing; agent; capable; forming; binding; pair; detectable; marker; attached; contacted; solid; support; agents; immobilized; acids; separated; detected; kit; performing; detectable marker; probe including; solid support; complexing agents; acid sequence; nucleic acid; acid sequences; nucleic acids; target nucleic; complexing agent; hybridization probe; hybridization probes; agent capable; binding pair; target nuclei; competitive hybridization; /435/999/

Citation Formats


                    Lucas, Joe N, Straume, Tore, and Bogen, Kenneth T. Detection and isolation of nucleic acid sequences using competitive hybridization probes.  United States: N. p., 1997. 
        Web.

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                    Lucas, Joe N, Straume, Tore, & Bogen, Kenneth T. Detection and isolation of nucleic acid sequences using competitive hybridization probes.  United States.

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                    Lucas, Joe N, Straume, Tore, and Bogen, Kenneth T. Wed .  
        "Detection and isolation of nucleic acid sequences using competitive hybridization probes".  United States.  https://www.osti.gov/servlets/purl/870890.

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@article{osti_870890,

  title        = {Detection and isolation of nucleic acid sequences using competitive hybridization probes},

  author       = {Lucas, Joe N and Straume, Tore and Bogen, Kenneth T},

  abstractNote = {A method for detecting a target nucleic acid sequence in a sample is provided using hybridization probes which competitively hybridize to a target nucleic acid. According to the method, a target nucleic acid sequence is hybridized to first and second hybridization probes which are complementary to overlapping portions of the target nucleic acid sequence, the first hybridization probe including a first complexing agent capable of forming a binding pair with a second complexing agent and the second hybridization probe including a detectable marker. The first complexing agent attached to the first hybridization probe is contacted with a second complexing agent, the second complexing agent being attached to a solid support such that when the first and second complexing agents are attached, target nucleic acid sequences hybridized to the first hybridization probe become immobilized on to the solid support. The immobilized target nucleic acids are then separated and detected by detecting the detectable marker attached to the second hybridization probe. A kit for performing the method is also provided.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1997},

  month        = {1}

}

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Works referenced in this record:

Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds
journal, May 1988

Chu, Barbara C. F.; Orgel, Leslie E.
Nucleic Acids Research, Vol. 16, Issue 9, p. 3671-3691
https://doi.org/10.1093/nar/16.9.3671

Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge
journal, January 1978

Sodja, Ann; Davidson, Norman
Nucleic Acids Research, Vol. 5, Issue 2, p. 358-401
https://doi.org/10.1093/nar/5.2.385

Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
journal, November 1981

Langer, P. R.; Waldrop, A. A.; Ward, D. C.
Proceedings of the National Academy of Sciences, Vol. 78, Issue 11, p. 6633-6637
https://doi.org/10.1073/pnas.78.11.6633

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Patent Lucas, Joe N [San Ramon, CA]; Straume, Tore [Tracy, CA]; Bogen, Kenneth T [Walnut Creek, CA]

A kit is provided for detecting a target nucleic acid sequence in a sample, the kit comprising: a first hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a first portion of the target sequence, the first hybridization probe including a first complexing agent for forming a binding pair with a second complexing agent; and a second hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a second portion of the target sequence to which the first hybridization probe does not selectively hybridize, the second hybridizationmore » « less
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A method for detecting a target nucleic acid sequence in a sample is provided using hybridization probes which competitively hybridize to a target nucleic acid. According to the method, a target nucleic acid sequence is hybridized to first and second hybridization probes which are complementary to overlapping portions of the target nucleic acid sequence, the first hybridization probe including a first complexing agent capable of forming a binding pair with a second complexing agent and the second hybridization probe including a detectable marker. The first complexing agent attached to the first hybridization probe is contacted with a second complexing agent,more » « less
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A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second nucleic acid sequence type (e.g., from a second chromosome), the presence of the first and the second nucleic acid sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. In the method, immobilization of a first hybridization probe is used to isolate a first set of nucleicmore » « less
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Similar Records

Title: Detection and isolation of nucleic acid sequences using competitive hybridization probes

Abstract

Citation Formats

Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds journal, May 1988

Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge journal, January 1978

Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. journal, November 1981

Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds
journal, May 1988

Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge
journal, January 1978

Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
journal, November 1981