skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Flow cytometer jet monitor system

Abstract

A direct jet monitor illuminates the jet of a flow cytometer in a monitor wavelength band which is substantially separate from the substance wavelength band. When a laser is used to cause fluorescence of the substance, it may be appropriate to use an infrared source to illuminate the jet and thus optically monitor the conditions within the jet through a CCD camera or the like. This optical monitoring may be provided to some type of controller or feedback system which automatically changes either the horizontal location of the jet, the point at which droplet separation occurs, or some other condition within the jet in order to maintain optimum conditions. The direct jet monitor may be operated simultaneously with the substance property sensing and analysis system so that continuous monitoring may be achieved without interfering with the substance data gathering and may be configured so as to allow the front of the analysis or free fall area to be unobstructed during processing.

Inventors:
 [1]
  1. Seattle, WA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
870824
Patent Number(s):
5602039
Assignee:
University of Washington ()
DOE Contract Number:  
FG06-93ER61662
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
flow; cytometer; jet; monitor; direct; illuminates; wavelength; band; substantially; separate; substance; laser; fluorescence; appropriate; infrared; source; illuminate; optically; conditions; ccd; camera; optical; monitoring; provided; type; controller; feedback; automatically; changes; horizontal; location; droplet; separation; occurs; condition; maintain; optimum; operated; simultaneously; property; sensing; analysis; continuous; achieved; interfering; data; gathering; configured; allow; front; free; fall; unobstructed; processing; continuous monitoring; ccd camera; data gathering; flow cytometer; wavelength band; separation occurs; substantially separate; jet monitor; optical monitor; infrared source; optically monitor; /436/209/250/356/422/435/

Citation Formats

Van den Engh, Ger. Flow cytometer jet monitor system. United States: N. p., 1997. Web.
Van den Engh, Ger. Flow cytometer jet monitor system. United States.
Van den Engh, Ger. Wed . "Flow cytometer jet monitor system". United States. https://www.osti.gov/servlets/purl/870824.
@article{osti_870824,
title = {Flow cytometer jet monitor system},
author = {Van den Engh, Ger},
abstractNote = {A direct jet monitor illuminates the jet of a flow cytometer in a monitor wavelength band which is substantially separate from the substance wavelength band. When a laser is used to cause fluorescence of the substance, it may be appropriate to use an infrared source to illuminate the jet and thus optically monitor the conditions within the jet through a CCD camera or the like. This optical monitoring may be provided to some type of controller or feedback system which automatically changes either the horizontal location of the jet, the point at which droplet separation occurs, or some other condition within the jet in order to maintain optimum conditions. The direct jet monitor may be operated simultaneously with the substance property sensing and analysis system so that continuous monitoring may be achieved without interfering with the substance data gathering and may be configured so as to allow the front of the analysis or free fall area to be unobstructed during processing.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

Patent:

Save / Share: