Flow cytometer jet monitor system

Van den Engh, Ger

Title: Flow cytometer jet monitor system

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Abstract

A direct jet monitor illuminates the jet of a flow cytometer in a monitor wavelength band which is substantially separate from the substance wavelength band. When a laser is used to cause fluorescence of the substance, it may be appropriate to use an infrared source to illuminate the jet and thus optically monitor the conditions within the jet through a CCD camera or the like. This optical monitoring may be provided to some type of controller or feedback system which automatically changes either the horizontal location of the jet, the point at which droplet separation occurs, or some other condition within the jet in order to maintain optimum conditions. The direct jet monitor may be operated simultaneously with the substance property sensing and analysis system so that continuous monitoring may be achieved without interfering with the substance data gathering and may be configured so as to allow the front of the analysis or free fall area to be unobstructed during processing.

Inventors:

Van den Engh, Ger ^[1]

Seattle, WA

Issue Date:: 1997-01-01

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

OSTI Identifier:: 870824

Patent Number(s):: 5602039

Assignee:: University of Washington ()

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N15/1404 - {Fluid conditioning in flow cytometers, e.g. flow cells
G01N2015/149 - {Sorting the particles}
G01N2015/1406 - {Control of droplet point}

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DOE Contract Number:: FG06-93ER61662

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: flow; cytometer; jet; monitor; direct; illuminates; wavelength; band; substantially; separate; substance; laser; fluorescence; appropriate; infrared; source; illuminate; optically; conditions; ccd; camera; optical; monitoring; provided; type; controller; feedback; automatically; changes; horizontal; location; droplet; separation; occurs; condition; maintain; optimum; operated; simultaneously; property; sensing; analysis; continuous; achieved; interfering; data; gathering; configured; allow; front; free; fall; unobstructed; processing; continuous monitoring; ccd camera; data gathering; flow cytometer; wavelength band; separation occurs; substantially separate; jet monitor; optical monitor; infrared source; optically monitor; /436/209/250/356/422/435/

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                    Van den Engh, Ger. Flow cytometer jet monitor system.  United States: N. p., 1997. 
        Web.

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                    Van den Engh, Ger. Flow cytometer jet monitor system.  United States.

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                    Van den Engh, Ger. Wed .  
        "Flow cytometer jet monitor system".  United States.  https://www.osti.gov/servlets/purl/870824.

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@article{osti_870824,

  title        = {Flow cytometer jet monitor system},

  author       = {Van den Engh, Ger},

  abstractNote = {A direct jet monitor illuminates the jet of a flow cytometer in a monitor wavelength band which is substantially separate from the substance wavelength band. When a laser is used to cause fluorescence of the substance, it may be appropriate to use an infrared source to illuminate the jet and thus optically monitor the conditions within the jet through a CCD camera or the like. This optical monitoring may be provided to some type of controller or feedback system which automatically changes either the horizontal location of the jet, the point at which droplet separation occurs, or some other condition within the jet in order to maintain optimum conditions. The direct jet monitor may be operated simultaneously with the substance property sensing and analysis system so that continuous monitoring may be achieved without interfering with the substance data gathering and may be configured so as to allow the front of the analysis or free fall area to be unobstructed during processing.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1997},

  month        = {1}

}

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