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Title: Recombinant glucose uptake system

Abstract

Recombinant organisms are disclosed that contain a pathway for glucose uptake other than the pathway normally utilized by the host cell. In particular, the host cell is one in which glucose transport into the cell normally is coupled to PEP production. This host cell is transformed so that it uses an alternative pathway for glucose transport that is not coupled to PEP production. In a preferred embodiment, the host cell is a bacterium other than Z. mobilis that has been transformed to contain the glf and glk genes of Z. mobilis. By uncoupling glucose transport into the cell from PEP utilization, more PEP is produced for synthesis of products of commercial importance from a given quantity of biomass supplied to the host cells.

Inventors:
 [1];  [2];  [3]
  1. Gainesville, FL
  2. Groede, NL
  3. Delft, NL
Issue Date:
Research Org.:
University of Florida (Gainesville, FL)
OSTI Identifier:
870823
Patent Number(s):
5602030
Assignee:
University of Florida (Gainesville, FL)
DOE Contract Number:  
FG05-86ER13574
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
recombinant; glucose; uptake; organisms; disclosed; contain; pathway; normally; utilized; host; cell; particular; transport; coupled; pep; production; transformed; alternative; preferred; embodiment; bacterium; z; mobilis; glf; glk; genes; uncoupling; utilization; produced; synthesis; products; commercial; importance; quantity; biomass; supplied; cells; preferred embodiment; host cells; host cell; recombinant organisms; glucose uptake; /435/

Citation Formats

Ingrahm, Lonnie O, Snoep, Jacob L, and Arfman, Nico. Recombinant glucose uptake system. United States: N. p., 1997. Web.
Ingrahm, Lonnie O, Snoep, Jacob L, & Arfman, Nico. Recombinant glucose uptake system. United States.
Ingrahm, Lonnie O, Snoep, Jacob L, and Arfman, Nico. Wed . "Recombinant glucose uptake system". United States. https://www.osti.gov/servlets/purl/870823.
@article{osti_870823,
title = {Recombinant glucose uptake system},
author = {Ingrahm, Lonnie O and Snoep, Jacob L and Arfman, Nico},
abstractNote = {Recombinant organisms are disclosed that contain a pathway for glucose uptake other than the pathway normally utilized by the host cell. In particular, the host cell is one in which glucose transport into the cell normally is coupled to PEP production. This host cell is transformed so that it uses an alternative pathway for glucose transport that is not coupled to PEP production. In a preferred embodiment, the host cell is a bacterium other than Z. mobilis that has been transformed to contain the glf and glk genes of Z. mobilis. By uncoupling glucose transport into the cell from PEP utilization, more PEP is produced for synthesis of products of commercial importance from a given quantity of biomass supplied to the host cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

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