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Title: Multiplexed capillary electrophoresis system

Abstract

The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

Inventors:
 [1];  [2];  [1];  [1];  [1]
  1. Ames, IA
  2. Silver Spring, MD
Issue Date:
Research Org.:
Ames Laboratory (AMES), Ames, IA; Iowa State University, Ames, IA (US)
OSTI Identifier:
870724
Patent Number(s):
5582705
Application Number:
08/444,565
Assignee:
Iowa State University Research Foundation, Inc. (Ames, IA)
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
multiplexed; capillary; electrophoresis; provides; side-entry; optical; excitation; geometry; charge-injection; device; optically; coupled; capillaries; array; interior; imaged; pixel; sanger-type; 4-label; dna; sequencing; reactions; nucleotide; identification; base; calling; improved; long-pass; filters; split; fluorescence; emission; channels; binary; poly; ethyleneoxide; matrix; electrophoretic; separations; multiplexed capillary; pass filters; capillary electrophoresis; dna sequencing; optically coupled; fluorescence emission; pass filter; sequencing reactions; side-entry optical; split fluorescence; base calling; binary poly; long-pass filters; optical excitation; nucleotide identification; charge-injection device; electrophoretic separations; injection device; emission channels; excitation geometry; electrophoretic separation; sequencing reaction; /204/

Citation Formats

Yeung, Edward S, Chang, Huan-Tsang, Fung, Eliza N, Li, Qingbo, and Lu, Xiandan. Multiplexed capillary electrophoresis system. United States: N. p., 1996. Web.
Yeung, Edward S, Chang, Huan-Tsang, Fung, Eliza N, Li, Qingbo, & Lu, Xiandan. Multiplexed capillary electrophoresis system. United States.
Yeung, Edward S, Chang, Huan-Tsang, Fung, Eliza N, Li, Qingbo, and Lu, Xiandan. Tue . "Multiplexed capillary electrophoresis system". United States. https://www.osti.gov/servlets/purl/870724.
@article{osti_870724,
title = {Multiplexed capillary electrophoresis system},
author = {Yeung, Edward S and Chang, Huan-Tsang and Fung, Eliza N and Li, Qingbo and Lu, Xiandan},
abstractNote = {The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {12}
}

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