Laser-induced differential normalized fluorescence method for cancer diagnosis
Abstract
An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample.
- Inventors:
-
- Knoxville, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 870708
- Patent Number(s):
- 5579773
- Assignee:
- Martin Marietta Energy Systems, Inc. (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- laser-induced; differential; normalized; fluorescence; method; cancer; diagnosis; apparatus; disclosed; diagnostic; steps; irradiating; tissue; sample; monochromatic; excitation; light; producing; spectrum; emission; radiation; generated; interaction; dividing; intensity; wavelength; integrated; produce; mathematical; difference; average; value; reference; set; spectra; correspond; normal; tissues; calculated; provides; amplifying; changes; weak; signals; malignant; improved; analysis; correlated; specific; condition; laser-induced fluorescence; tissue sample; diagnostic method; radiation generated; excitation light; induced fluorescence; fluorescence spectrum; normal tissue; fluorescence method; cancer diagnosis; malignant tissue; /600/
Citation Formats
Vo-Dinh, Tuan, Panjehpour, Masoud, and Overholt, Bergein F. Laser-induced differential normalized fluorescence method for cancer diagnosis. United States: N. p., 1996.
Web.
Vo-Dinh, Tuan, Panjehpour, Masoud, & Overholt, Bergein F. Laser-induced differential normalized fluorescence method for cancer diagnosis. United States.
Vo-Dinh, Tuan, Panjehpour, Masoud, and Overholt, Bergein F. Mon .
"Laser-induced differential normalized fluorescence method for cancer diagnosis". United States. https://www.osti.gov/servlets/purl/870708.
@article{osti_870708,
title = {Laser-induced differential normalized fluorescence method for cancer diagnosis},
author = {Vo-Dinh, Tuan and Panjehpour, Masoud and Overholt, Bergein F},
abstractNote = {An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1996},
month = {Mon Jan 01 00:00:00 EST 1996}
}