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Title: DNA fragment sizing and sorting by laser-induced fluorescence

A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.
Inventors:
 [1];  [2];  [2];  [2];  [2]
  1. (Angier, NC)
  2. (Los Alamos, NM)
Issue Date:
OSTI Identifier:
870612
Assignee:
Regents of Univ. of California (Alameda, CA) LANL
Patent Number(s):
US 5558998
Contract Number:
W-7405-ENG-36
Research Org:
Los Alamos National Laboratory (LANL), Los Alamos, NM
Country of Publication:
United States
Language:
English
Subject:
dna; fragment; sizing; sorting; laser-induced; fluorescence; method; provided; fragments; speed; detection; systems; flow; cytometry; determine; unique; characteristics; pieces; sample; characterization; piece; fragmented; preselected; sites; produce; plurality; resulting; treated; dye; effective; stain; stoichiometrically; stained; examined; generate; output; functionally; related; nucleotides; embodiment; intensity; emissions; linearly; length; distribution; sizes; forms; forensic; applications; laser-induced fluorescence; detection systems; functionally related; dna fragments; fluorescence emission; flow cytometry; dna fragment; unique characteristics; induced fluorescence; fluorescence emissions; linearly related; fragment sizing; unique characteristic; /435/436/

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