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Title: Method for promoting specific alignment of short oligonucleotides on nucleic acids

Disclosed is a method for promoting specific alignment of short oligonucleotides on a nucleic acid polymer. The nucleic acid polymer is incubated in a solution containing a single-stranded DNA-binding protein and a plurality of oligonucleotides which are perfectly complementary to distinct but adjacent regions of a predetermined contiguous nucleotide sequence in the nucleic acid polymer. The plurality of oligonucleotides anneal to the nucleic acid polymer to form a contiguous region of double stranded nucleic acid. Specific application of the methods disclosed include priming DNA synthesis and template-directed ligation.
Inventors:
 [1];  [2];  [3]
  1. (Stony Brook, NY)
  2. (Coram, NY)
  3. (Bellport, NY)
Issue Date:
OSTI Identifier:
870573
Assignee:
Associated Universities, Inc. (Washington, DC) BNL
Patent Number(s):
US 5547843
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY
Country of Publication:
United States
Language:
English
Subject:
method; promoting; specific; alignment; oligonucleotides; nucleic; acids; disclosed; acid; polymer; incubated; solution; containing; single-stranded; dna-binding; protein; plurality; perfectly; complementary; distinct; adjacent; regions; predetermined; contiguous; nucleotide; sequence; anneal; form; region; double; stranded; application; methods; priming; dna; synthesis; template-directed; ligation; acid polymer; stranded dna; dna synthesis; binding protein; nucleotide sequence; solution containing; nucleic acid; nucleic acids; specific application; single-stranded dna; double stranded; specific alignment; promoting specific; stranded nucleic; /435/