DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Method for promoting specific alignment of short oligonucleotides on nucleic acids

Abstract

Disclosed is a method for promoting specific alignment of short oligonucleotides on a nucleic acid polymer. The nucleic acid polymer is incubated in a solution containing a single-stranded DNA-binding protein and a plurality of oligonucleotides which are perfectly complementary to distinct but adjacent regions of a predetermined contiguous nucleotide sequence in the nucleic acid polymer. The plurality of oligonucleotides anneal to the nucleic acid polymer to form a contiguous region of double stranded nucleic acid. Specific application of the methods disclosed include priming DNA synthesis and template-directed ligation.

Inventors:
 [1];  [2];  [3]
  1. Stony Brook, NY
  2. Coram, NY
  3. Bellport, NY
Issue Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
OSTI Identifier:
870573
Patent Number(s):
5547843
Assignee:
Associated Universities, Inc. (Washington, DC)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; promoting; specific; alignment; oligonucleotides; nucleic; acids; disclosed; acid; polymer; incubated; solution; containing; single-stranded; dna-binding; protein; plurality; perfectly; complementary; distinct; adjacent; regions; predetermined; contiguous; nucleotide; sequence; anneal; form; region; double; stranded; application; methods; priming; dna; synthesis; template-directed; ligation; acid polymer; stranded dna; dna synthesis; binding protein; nucleotide sequence; solution containing; nucleic acid; nucleic acids; specific application; single-stranded dna; double stranded; specific alignment; promoting specific; stranded nucleic; /435/

Citation Formats

Studier, F William, Kieleczawa, Jan, and Dunn, John J. Method for promoting specific alignment of short oligonucleotides on nucleic acids. United States: N. p., 1996. Web.
Studier, F William, Kieleczawa, Jan, & Dunn, John J. Method for promoting specific alignment of short oligonucleotides on nucleic acids. United States.
Studier, F William, Kieleczawa, Jan, and Dunn, John J. Mon . "Method for promoting specific alignment of short oligonucleotides on nucleic acids". United States. https://www.osti.gov/servlets/purl/870573.
@article{osti_870573,
title = {Method for promoting specific alignment of short oligonucleotides on nucleic acids},
author = {Studier, F William and Kieleczawa, Jan and Dunn, John J},
abstractNote = {Disclosed is a method for promoting specific alignment of short oligonucleotides on a nucleic acid polymer. The nucleic acid polymer is incubated in a solution containing a single-stranded DNA-binding protein and a plurality of oligonucleotides which are perfectly complementary to distinct but adjacent regions of a predetermined contiguous nucleotide sequence in the nucleic acid polymer. The plurality of oligonucleotides anneal to the nucleic acid polymer to form a contiguous region of double stranded nucleic acid. Specific application of the methods disclosed include priming DNA synthesis and template-directed ligation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}

Works referenced in this record:

Improved yields of long PCR products using gene 32 protein
journal, January 1990


Sequencing of megabase plus DNA by hybridization: Theory of the method
journal, February 1989


A library of 3342 useful nonamer primers for genome sequencing
journal, January 1990