Gene coding for the E1 endoglucanase
Abstract
The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol.
- Inventors:
-
- Denver, CO
- Littleton, CO
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 870513
- Patent Number(s):
- 5536655
- Assignee:
- Midwest Research Institute (Kansas City, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-83CH10093
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- coding; e1; endoglucanase; encoding; acidothermus; cellulolyticus; cloned; expressed; heterologous; microorganisms; modified; enzyme; produced; variants; useful; hydrolyzing; cellulose; sugars; simultaneous; fermentation; alcohol; e1 endoglucanase; acidothermus cellulolyticus; modified e1; endoglucanase enzyme; encoding acidothermus; cellulolyticus e1; /435/999/
Citation Formats
Thomas, Steven R, Laymon, Robert A, and Himmel, Michael E. Gene coding for the E1 endoglucanase. United States: N. p., 1996.
Web.
Thomas, Steven R, Laymon, Robert A, & Himmel, Michael E. Gene coding for the E1 endoglucanase. United States.
Thomas, Steven R, Laymon, Robert A, and Himmel, Michael E. Mon .
"Gene coding for the E1 endoglucanase". United States. https://www.osti.gov/servlets/purl/870513.
@article{osti_870513,
title = {Gene coding for the E1 endoglucanase},
author = {Thomas, Steven R and Laymon, Robert A and Himmel, Michael E},
abstractNote = {The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}
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Works referenced in this record:
Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria
journal, July 1986
- MOHAGHEGHI, A.; GROHMANN, K.; HIMMEL, M.
- International Journal of Systematic Bacteriology, Vol. 36, Issue 3, p. 435-443