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Title: Method for nucleic acid hybridization using single-stranded DNA binding protein

Abstract

Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.

Inventors:
 [1];  [2]
  1. Cambridge, MA
  2. Chestnut Hill, MA
Issue Date:
Research Org.:
Harvard University
OSTI Identifier:
870501
Patent Number(s):
5534407
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-88ER60688
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; nucleic; acid; hybridization; single-stranded; dna; binding; protein; detecting; presence; specific; sequence; population; sequences; probe; hybridizes; contacting; sample; potentially; including; hybridizing; conditions; provided; amount; stimulates; renaturation; dilute; solution; weeks; 500; fold; 60; preferably; absence; nucleotide; triphosphates; stranded dna; acid hybridization; dilute solution; binding protein; acid sequence; nucleic acid; acid sequences; acid probe; specific nucleic; single-stranded dna; dna binding; /435/

Citation Formats

Tabor, Stanley, and Richardson, Charles C. Method for nucleic acid hybridization using single-stranded DNA binding protein. United States: N. p., 1996. Web.
Tabor, Stanley, & Richardson, Charles C. Method for nucleic acid hybridization using single-stranded DNA binding protein. United States.
Tabor, Stanley, and Richardson, Charles C. Mon . "Method for nucleic acid hybridization using single-stranded DNA binding protein". United States. https://www.osti.gov/servlets/purl/870501.
@article{osti_870501,
title = {Method for nucleic acid hybridization using single-stranded DNA binding protein},
author = {Tabor, Stanley and Richardson, Charles C},
abstractNote = {Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}

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Works referenced in this record:

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