Title: Method for nucleic acid hybridization using single-stranded DNA binding protein

Abstract

Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.

Inventors:

Tabor, Stanley ^[1]; Richardson, Charles C ^[2]

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1. Cambridge, MA
2. Chestnut Hill, MA

Issue Date:

Mon Jan 01 00:00:00 EST 1996

Research Org.:

Harvard University

OSTI Identifier:

870501

Patent Number(s):

5534407

Assignee:

President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6813 - Hybridisation assays
C12Q1/6832 - Enhancement of hybridisation reaction

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DOE Contract Number:  

FG02-88ER60688

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

method; nucleic; acid; hybridization; single-stranded; dna; binding; protein; detecting; presence; specific; sequence; population; sequences; probe; hybridizes; contacting; sample; potentially; including; hybridizing; conditions; provided; amount; stimulates; renaturation; dilute; solution; weeks; 500; fold; 60; preferably; absence; nucleotide; triphosphates; stranded dna; acid hybridization; dilute solution; binding protein; acid sequence; nucleic acid; acid sequences; acid probe; specific nucleic; single-stranded dna; dna binding; /435/