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Title: Apparatus and method for polymer synthesis using arrays

Abstract

A polymer synthesis apparatus (20) for building a polymer chain including a head assembly (21) having an array of nozzles (22) with each nozzle coupled to a reservoir (23) of liquid reagent (24), and a base assembly (25) having an array of reaction wells (26). A transport mechanism (27) aligns the reaction wells (26) and selected nozzles (22) for deposition of the liquid reagent (24) into selected reaction wells (26). A sliding seal (30) is positioned between the head assembly (21) and the base assembly (25) to form a common chamber (31) enclosing both the reaction well (26) and the nozzles (22) therein. A gas inlet (70) into the common chamber (31), upstream from the nozzles (22), and a gas outlet (71) out of the common chamber (31), downstream from the nozzles (22), sweeps the common chamber (31) of toxic fumes emitted by the reagents. Each reaction well ( 26) includes an orifice (74) extending into the well (26) which is of a size and dimension to form a capillary liquid seal to retain the reagent solution (76) in the well (26) for polymer chain growth therein. A pressure regulating device (82 ) is provided for controlling a pressure differential,more » between a first gas pressure exerted on the reaction well (26) and a second gas pressure exerted on an exit (80) of the orifice, such that upon the pressure differential exceeding a predetermined amount, the reagent solution (76) is expelled from the well (26) through the orifice (74). A method of synthesis of a polymer chain in a synthesis apparatus (20) is also included.

Inventors:
 [1]
  1. San Francisco, CA
Issue Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
OSTI Identifier:
870484
Patent Number(s):
5529756
Assignee:
Board of Trustees of Leland Stanford Junior University (Palo Alto, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07B - GENERAL METHODS OF ORGANIC CHEMISTRY
DOE Contract Number:  
DOE LBL 4437-10
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
apparatus; method; polymer; synthesis; arrays; 20; building; chain; including; head; assembly; 21; array; nozzles; 22; nozzle; coupled; reservoir; 23; liquid; reagent; 24; base; 25; reaction; 26; transport; mechanism; 27; aligns; selected; deposition; sliding; seal; 30; positioned; form; common; chamber; 31; enclosing; therein; gas; inlet; 70; upstream; outlet; 71; downstream; sweeps; toxic; fumes; emitted; reagents; orifice; 74; extending; size; dimension; capillary; retain; solution; 76; growth; pressure; regulating; device; 82; provided; controlling; differential; exerted; exit; 80; exceeding; predetermined; amount; expelled; included; selected reaction; transport mechanism; liquid seal; polymer synthesis; pressure regulating; liquid reagent; regulating device; synthesis apparatus; head assembly; gas outlet; gas inlet; pressure differential; gas pressure; reagent solution; common chamber; polymer chain; predetermined amount; pressure exerted; chain growth; capillary liquid; nozzle coupled; chain including; growth therein; /422/

Citation Formats

Brennan, Thomas M. Apparatus and method for polymer synthesis using arrays. United States: N. p., 1996. Web.
Brennan, Thomas M. Apparatus and method for polymer synthesis using arrays. United States.
Brennan, Thomas M. Mon . "Apparatus and method for polymer synthesis using arrays". United States. https://www.osti.gov/servlets/purl/870484.
@article{osti_870484,
title = {Apparatus and method for polymer synthesis using arrays},
author = {Brennan, Thomas M},
abstractNote = {A polymer synthesis apparatus (20) for building a polymer chain including a head assembly (21) having an array of nozzles (22) with each nozzle coupled to a reservoir (23) of liquid reagent (24), and a base assembly (25) having an array of reaction wells (26). A transport mechanism (27) aligns the reaction wells (26) and selected nozzles (22) for deposition of the liquid reagent (24) into selected reaction wells (26). A sliding seal (30) is positioned between the head assembly (21) and the base assembly (25) to form a common chamber (31) enclosing both the reaction well (26) and the nozzles (22) therein. A gas inlet (70) into the common chamber (31), upstream from the nozzles (22), and a gas outlet (71) out of the common chamber (31), downstream from the nozzles (22), sweeps the common chamber (31) of toxic fumes emitted by the reagents. Each reaction well ( 26) includes an orifice (74) extending into the well (26) which is of a size and dimension to form a capillary liquid seal to retain the reagent solution (76) in the well (26) for polymer chain growth therein. A pressure regulating device (82 ) is provided for controlling a pressure differential, between a first gas pressure exerted on the reaction well (26) and a second gas pressure exerted on an exit (80) of the orifice, such that upon the pressure differential exceeding a predetermined amount, the reagent solution (76) is expelled from the well (26) through the orifice (74). A method of synthesis of a polymer chain in a synthesis apparatus (20) is also included.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1996},
month = {Mon Jan 01 00:00:00 EST 1996}
}

Works referenced in this record:

Light-directed, spatially addressable parallel chemical synthesis
journal, February 1991


Direct cleavage of peptides from a solid support into aqueous buffer. Application in simultaneous multiple peptide synthesis
journal, November 1991


An oligonucleotide hybridization approach to DNA sequencing
journal, October 1989