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Title: Electrorheological crystallization of proteins and other molecules

An electrorheological crystalline mass of a molecule is formed by dispersing the molecule in a dispersion fluid and subjecting the molecule dispersion to a uniform electrical field for a period of time during which time an electrorheological crystalline mass is formed. Molecules that may be used to form an electrorheological crystalline mass include any organic or inorganic molecule which has a permanent dipole and/or which is capable of becoming an induced dipole in the presence of an electric field. The molecules used to form the electrorheological crystalline mass are preferably macromolecules, such as biomolecules, such as proteins, nucleic acids, carbohydrates, lipoproteins and viruses. Molecules are crystallized by a method in which an electric field is maintained for a period of time after the electrorheological crystalline mass has formed during which time at least some of the molecules making up the electrorheological crystalline mass form a crystal lattice. The three dimensional structure of a molecule is determined by a method in which an electrorheological crystalline mass of the molecule is formed, an x-ray diffraction pattern of the electrorheological crystalline mass is obtained and the three dimensional structure of the molecule is calculated from the x-ray diffraction pattern.
Inventors:
 [1];  [2]
  1. (Lafayette, CA)
  2. (Dublin, CA)
Issue Date:
OSTI Identifier:
870450
Assignee:
Regents of University of California (Oakland, CA) LLNL
Patent Number(s):
US 5525198
Contract Number:
W-7405-ENG-48
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Country of Publication:
United States
Language:
English
Subject:
electrorheological; crystallization; proteins; molecules; crystalline; mass; molecule; formed; dispersing; dispersion; fluid; subjecting; uniform; electrical; field; period; time; form; organic; inorganic; permanent; dipole; capable; becoming; induced; presence; electric; preferably; macromolecules; biomolecules; nucleic; acids; carbohydrates; lipoproteins; viruses; crystallized; method; maintained; crystal; lattice; dimensional; structure; determined; x-ray; diffraction; pattern; obtained; calculated; electrical field; x-ray diffraction; electric field; nucleic acid; nucleic acids; crystal lattice; diffraction pattern; organic molecule; dimensional structure; uniform electrical; uniform electric; form electrical; /204/205/378/435/